Alpha-Select Bacteriophage T1-Resistant Gold Efficiency - Discontinued


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α-Select Bacteriophage T1-Resistant Gold Competent Cells are ideal for generating high efficiency libraries cloning with limited amounts of DNA.

Features & Benefits

  • Reduced recombination of cloned DNA -designed to accommodates larger plasmids
  • High efficiency -available in ≥109 cfu/μg, perfect for library construction
  • Easy screening -with blue/white color selection
  • Convenient size -small aliquots reduce waste

Applications

  • Blue/white color screening
  • Construction of gene banks
  • Generation of cDNA libraries using plasmid-derived vectors
  • High quality plasmid preparation
  • Hosting H13mp cloning vectors

Description

α-Select Bacteriophage T1-Resistant Gold Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. α-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

Many laboratories have experienced bacteriophage T1 outbreaks, as T1 attacks E. coli and spreads rapidly. α-Select Bacteriophage T1-Resistant Gold Competent Cells are designed to protect samples from bacteriophage infection.

pUC19 DNA is also provided as a positive control.


Notes

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-


Components

Reagent

BIO-85030

Chemically Competent Cells

20 x 50µl

pUC19 control vector (10pg/µl)

50µl


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on Dry Ice or Blue Ice.







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