BIOLASE™ PCR Kit - Discontinued


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The BIOLASE™ PCR Kit contains all the necessary components to perform PCR assays on a wide range of DNA templates. In addition to dNTPs, the PCR Kit is based on our widely used BIOLASE DNA Polymerase, which achieves dependable results.

Features & Benefits

  • Good standard Taq polymerase - ideal for setting up new procedures
  • Easy to use - designed for fast and easy optimization of PCR applications
  • High quality reagents - contains ultra-pure dNTPs manufactured by Bioline
  • Flexible format - upplied with 2x PolyMate Additive for difficult or "dirty" templates

Applications

  • Routine PCR applications
  • Products suitable for TA cloning

Description

The BIOLASE™ PCR Kit contains all the necessary components to perform PCR assays on a wide range of DNA templates. The PCR Kit is based on our widely used BIOLASE DNA Polymerase and includes dNTPs to deliver dependable results.

BIOLASE DNA polymerase is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. BIOLASE is a robust preparation and consistently delivers high yields with minimal background.

BIOLASE possesses 5´-3´ exonuclease activity and leaves an ´-A´- overhang such that the primer extension product is suitable for effective integration into TA cloning vectors.

The Kit Includes buffer, MgCl2 solution, ultra-pure dNTP Mix and 2x PolyMate Additive for "dirty" or difficult templates with GC or AT-rich DNA, repetitive sequences, or difficult melting profiles).


Notes

BIOLASE is a trademark of Bioline.


Components

  • BIOLASE™ DNA Polymerase
  • 10x NH4 Buffer
  • 50mM MgCl2 Solution
  • 10mM dNTP Mix
  • 2x PolyMate Additive

Concentration


5u/µl

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.







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