BioScript™ is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase possessing low RNase H activity and high sensitivity. BioScript is suitable for first-strand cDNA synthesis, cDNA library construction and the production of cDNA for PCR analysis of gene expression.
- Stable - for long genes and rare transcripts
- Highly sensitive - for enhanced cDNA yield
- High quality cDNA - ideal for PCR
- Broad temperature range - for RNA with secondary structure
BioScript™ is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability and is active over a wide range of temperatures with no loss of activity following 1 week at room temperature. Unlike the wild-type enzyme, BioScript possesses low RNase H activity, which results in enhanced yields (Fig. 1 & 2). In addition, BioScript is highly sensitive even when the amount of template is a limiting factor.
BioScript is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of cDNA for PCR analysis of gene expression. BioScript can be used with total RNA, mRNA or in-vitro transcribed RNA.
- First strand cDNA synthesis for PCR
- cDNA library construction
- mRNA 5’ end mapping by primer extension
- Dideoxynucleotide sequencing
- End-labelling of DNA
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Certification of Analysis (COAs)
- 5x Reaction Buffer
Storage & Stability
All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.
BioScript is shipped on dry/blue ice