| Sign In | Quick Order CART (0)
  • Ordering

    Cat. No.
    Size
    List Price*
    QTY
    BIO-21040
    500 Units
    $210.00
    +
    BIO-21060
    2500 Units
    $850.00
    +
    *To check your pricing please Login or contact us

    Description

    BIOTAQ is a highly purified, thermostable DNA polymerase offering high yield over a wide range of PCR templates and a good choice for routine PCR assays.

    BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

    Product Highlights

    • Good standard Taq polymerase - ideal for setting up new procedures
    • Easy to use - designed for easy optimization of PCR applications
    • Suitable for TA cloning - leaves 'A' overhang

    Product Description

    BIOTAQ™ is a purified thermostable DNA polymerase offering high yield over a wide range of PCR templates (fig. 1), and is a good choice for routine assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

    BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.

    Applications

    • Routine PCR applications
    • TA cloning
    Main

    PCR Selection Chart

    Select the best reagent for your research

    PCR Enzyme Guide

    Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Specification

    Components

    Reagent

    100 Units

    500 Units

    2500 Units

    BIOTAQ DNA Polymerase

    1 x 20 µL

    1 x 100 µL

    5 x 100 µL

    10x NH4 Reaction Buffer

    1 x 1.2 ml

    2 x 1.2 ml

    10 x 1.2 ml

    50 mM MgCl2 Solution

    1 x 1.2 ml

    1 x 1.2 ml

    5 x 1.2 ml

    Concentration

    5 u/μL

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    On Dry Ice or Blue Ice.

    One unit will incorporate 10 nmoles of dNTPs in 30 min at 72°C.


    BIOTAQ is a trademark of Bioline.



    FAQs

    Please see the following table for the appropriate error rates:

    BIOTAQ, IMMOLASE, MangoTaq, MyTaq DNA Polymerase
     1.1 x 10-4 base substitutions/bp (Tindall and Kunkel, 1988)
     2.4 x 10-5 frameshift mutations/bp (Tindall and Kunkel, 1988)
     2.1 x 10-4 errors/bp (Keohavang and Thilly, 1989)
     7.2 x 10-5 errors/bp (Ling et al., 1991)
     8.9 x 10-5 errors/bp (Cariello et al., 1991)
     2.0 x 10-5 errors/bp (Lundberg et al., 1991)
     1.1 x 10-4 errors/bp (Barnes, 1992)
    ACCUZYME  1.6 x 10-6 errors/base (Lundberg et al., 1991).
    VELOCITY  4.4 x 10-7 errors/base (Frey & Suppmann, 1995).


    BIOTAQ DNA Polymerase is a fast enzyme, which will extend fragments at 15-30 s/Kb, dependent on template amplified.

    Yes, BIOTAQ DNA Polymerase does not possess 3' to 5' exonuclease activity, and therefore leaves an A-overhang at the 3' end, thus making the PCR product suitable for integration into TA cloning vectors.

    BIOTAQ will comfortably amplify fragments of up to 5 kb with genomic templates.

    BIOTAQ DNA Polymerase will extend between 50-80°C, however optimal extension will occur at 72°C.

    BIOTAQ DNA Polymerase is supplied at 5 u/µL.

    At Bioline we pride ourselves in supplying high-quality polymerases to suit your requirements. To aid your selection of the most suited enzyme for your specific applications, please see our enzyme selection tool.

    All our polymerases are guaranteed for a period of 12 months from the date of purchase. These should be stored at -20°C during this time for optimal retention of activity.

    Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.

    PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

    Observation Recommended Solution(s)
    No or low PCR yield  Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.
     Primers degraded – check quality and age of the primers.
     Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting  concentration of 1.75 mM.
     Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both  primers have the same concentration.
     Template concentration too low – Increase concentration of template.
     Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.
    Multiple Bands  Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.
     Prepare master mixes on ice or use a heat-activated polymerase.
     For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.
    Smearing or artifacts  Template concentration too high. Prepare serial dilutions of template.
     Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.
     Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.
     Extension time too long. Reduce extension time in 0.5-1 minute increments.


    These terms refer to parameters to be considered when performing PCR and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial: Yield: The amount of DNA produced in a PCR reaction. Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand. Processivity: The length of time a polymerase is associated with the template and therefore the size of fragment which can be amplified. Specificity: A measure of the unwanted by-products generated in a reaction.

    One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C.

    Reviews

    "I have always used Bioline Taq polymerase from starting my PhD in 2000 up until ..."

    ""I assessed BIOTAQ for amplicon sequencing and found a high level of (obser ..."