BL21 ComboPack - Discontinued


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The BL21 Competent Cell Combo Pack provides everything you need for T7 promotor driven protein expression, whether you are setting up new protein expression experiments, or need to express a set of proteins with different properties.

Features & Benefits

  • High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning
  • Protease deficient - facilitates the isolation of intact recombinant proteins
  • T7 Promoter - IPTG induction for controled expression of target genes
  • Convenient size - small aliquots reduce waste

Applications

  • Setting up new protein expression experiments

Description

The BL21 Competent Cell Combo Pack provides everything you need for T7 promotor driven protein expression, whether you are setting up new protein expression experiments, or need to express a set of proteins with different properties.

These strains are lysogens of bacteriophage DE3, a lambda derivative containing the gene for T7 RNA polymerase under control of the lacUV5 promotor. Induction with IPTG allows production of T7 RNA Polymerase. This then directs the expression of the target gene located downstream of the T7 promotor in the expression vector. Each BL21 (DE3) strain provides varying degrees of regulation and expression control. The tightest regulation and expression control is achieved in the BL21(DE3)pLysE and BL21(DE3)pLysS strains, making them ideal for the expression of proteins that are toxic to E.coli. Recombinant proteins that are non-toxic to E. coli are generally expressed at higher levels in BL21 (DE3) cells than in BL21(DE3)pLysS or BL21 (DE3)pLysE.


Notes

BL21(DE3) Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3)

BL21(DE3)pLysS Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3)pLysS (CamR)

BL21(DE3)pLysE Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3)pLysE (CamR)


Components

Reagent

BIO-85035

BL21(DE3) Chemically Competent Cells

5 x 100µl

BL21(DE3)pLysS Chemically Competent Cells

5 x 100µl

BL21(DE3)pLysE Chemically Competent Cells

5 x 100µl

pUC19 control vector (100pg/µl)

100µl


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on Dry Ice or Blue Ice.







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