BL21 (DE3) - Discontinued


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Chemically competent E. coli cells suitable for transformation and protein expression. This strain contains the T7 RNA polymerase gene for controlled gene expression.

Features & Benefits

  • High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning
  • Protease deficient - facilitates the isolation of intact recombinant proteins
  • T7 Promoter - IPTG induction for high protein expression
  • Convenient size - small aliquots reduce waste

Applications

T7 promotor protein expression


Description

BL21(DE3) competent cells are chemically competent cells used for high-level protein expression with T7 RNA polymerase-based expression systems. The BL21(DE3) contains the T7 RNA polymerase gene, under the control of the lacUV5 promoter, integrated into the chromosome. IPTG is used to induce the expression of recombinant proteins cloned into vectors downstream of a T7 RNA promoter and transformed into the BL21(DE3) cells.


Notes

Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3)


Components

Reagent

BIO-85032

Chemically Competent Cells

10 x 100µl

pUC19 control vector (100pg/µl)

100µl


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on Dry Ice or Blue Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for BL21 (DE3) - Discontinued are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 3 COAs for BL21 (DE3) - Discontinued →





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