Bioline is proud to announce the latest addition to its family of SensiFAST Real-Time PCR products.
SensiFAST™ HRM Kit facilitates High Resolution Melt (HRM) curve analysis, enabling amplification and discrimination of even the most challenging sequence differences -such as class 4 SNPs- without sequence preference.
SensiFAST HRM is designed to deliver fast, accurate detection of gene mutations and SNPs and provides reliable and highly reproducible data on all commonly used real-time PCR instruments, especially the new generation of fast-cyclers. SensiFAST HRM does not require expensive labelled oligonucleotide probes and offers a cost effective alternative to traditional probe based genotyping methods.
Marco Calzavara, President of Bioline said:
For more information, please visit the SensiFAST™ HRM product page.
As no one can fail to have noticed by now, today is Valentine's Day, a day when we all go out of our way to show special affection for the ones we love.
So, this February, why not show some love for Bioline by telling us why you use your favourite Bioline reagents? In return we'll send you a free lab timer for your efforts.
But that's not all! Because love is a two way thing, we've set aside some iPod Shuffles to give away to the originators of any product testimonials that we use.
We know that many scientists find Bioline's high-quality reagents for molecular biology are the only ones that get the job done in-time, on-time, every time with their applications.
So, if this description fits you, you love your Bioline products and you'd like a free lab timer and a chance to win a 2GB iPod Shuffle, all you need to do is leave your testimonial as a comment and email us your contact details.
Or, if you prefer, email us directly with your quote and contact details and we'll do the rest.
We look forward to hearing from you! In the meantime, Happy Valentine's Day from all at Bioline: The PCR Company.
Keep up-to-date with cutting-edge developments in your particular area of research with Bioline Scholar Monthly, Bioline’s new resource for academic and industry researchers who want to stay ahead of the game.
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Learn best practice from laboratory scientists around the world who have already published successfully with Bioline’s range of industry-leading products – designed to accelerate breakthroughs in all major fields of life science.
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Bioline Scholar Monthly: January 2012 Round-Up
The first in our series of Bioline Scholar Monthly compilations focuses on the MyTaq™ DNA Polymerase Family.
Interesting set of papers for detecting the spread of novel genes from veterinary viruses.
Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011) - Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)
Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011) - Distribution and genetic diversity of porcine hokovirus in wild boars
Study of woodland population genetics by 454 shotgun pyrosequencing & PCR. Lead author of the paper, Dr Olivier Lepais, commented:
"We used MyTaq to screen for suitable microsatellite markers in simplex PCR. We found that MyTaq Mix gave great results and was very easy to work with. This ease of use was critical as the primer screening involved a high number of independent PCR mixes!"
Lepais, O. L. & Bacles, C. F. J. Hered. doi: 10.1093/jhered/esr062 (2011) - De Novo Discovery and Multiplexed Amplification of Microsatellite Markers for Black Alder (Alnus glutinosa) and Related Species Using SSR-Enriched Shotgun Pyrosequencing
Study of microsatellite markers to study the genetic diversity of lobsters in Australia and New Zealand.
Thomas, L. & Bell, J.J. Conserv. Gene. Resources doi: 10.1007/s12686-011-9537-x (2011) - Characterization of polymorphic microsatellite markers for the red rock lobster, Jasus edwardsii (Hutton 1875)
Study on new molecular genetics assay for investigating harmful algal blooms, Sydney Institute of Marine Sciences.
Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011) - sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters
Protocol on preparing ssDNA from PCR products with streptavidin magnetic beads.
Wilson, R. Nucleic Acid Therapeutics doi:10.1089/nat.2011.0322 (2011) - Preparation of Single-Stranded DNA from PCR Products with Streptavidin Magnetic Beads
Interesting new veterinary parasitology paper from the University of New South Wales, Sydney & Merial, a Sanofi Company.
Šlapeta, J., et al. Vet Parasitol. doi: 10.1016/j.vetpar.2011.03.035 (2011) - The cat flea (Ctenocephalides f. felis) is the dominant flea on domestic dogs and cats in Australian veterinary practices
Bacteria-triggered histone modification in a fungus showing previously undescribed evidence of Saga/Ada dependent histone acetylation triggered by prokaryotes.
Nützmann, H-W., et al. PNAS 108, 14282-14287 (2011) - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation
Neuroscience paper from Department of Cell & Molecular Physiology, Neuroscience Center & Medical School at University of North Carolina.
Buttermore, E.D., et al. J. Neurosci. 31(22), 8013-8024 (2011) - The Cytoskeletal Adaptor Protein Band 4.1B Is Required for the Maintenance of Paranodal Axoglial Septate Junctions in Myelinated Axons