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February 2012

BIOLINE Introduces SensiFAST™ HRM Kit for Gene Mutations & SNP Analysis

By Bioline: The PCR Company 21 February 2012 No comments

[caption id="attachment_394" align="alignleft" width="150" caption="SensiFAST HRM Kit - your first choice for HRM curve analysis"]SensiFAST HRM Kit, the first choice for HRM curve analysis

Bioline is proud to announce the latest addition to its family of SensiFAST Real-Time PCR products.

SensiFAST™ HRM Kit facilitates High Resolution Melt (HRM) curve analysis, enabling amplification and discrimination of even the most challenging sequence differences -such as class 4 SNPs- without sequence preference.

SensiFAST HRM is designed to deliver fast, accurate detection of gene mutations and SNPs and provides reliable and highly reproducible data on all commonly used real-time PCR instruments, especially the new generation of fast-cyclers. SensiFAST HRM does not require expensive labelled oligonucleotide probes and offers a cost effective alternative to traditional probe based genotyping methods.

Marco Calzavara, President of Bioline said:

"I am delighted to announce the release of SensiFAST HRM as a new member of our real-time PCR-based SensiFAST family. This new kit will enable researchers to cost-effectively scan DNA from a variety of biological sample types to detect the smallest genetic variations among samples."

For more information, please visit the SensiFAST™ HRM product page.

Show some love for Bioline, win an iPod Shuffle

By Bioline: The PCR Company 14 February 2012 No comments

Apple iPod Shuffle

As no one can fail to have noticed by now, today is Valentine's Day, a day when we all go out of our way to show special affection for the ones we love.

So, this February, why not show some love for Bioline by telling us why you use your favourite Bioline reagents? In return we'll send you a free lab timer for your efforts.

But that's not all! Because love is a two way thing, we've set aside some iPod Shuffles to give away to the originators of any product testimonials that we use.

We know that many scientists find Bioline's high-quality reagents for molecular biology are the only ones that get the job done in-time, on-time, every time with their applications.

Free Lab Timer

So, if this description fits you, you love your Bioline products and you'd like a free lab timer and a chance to win a 2GB iPod Shuffle, all you need to do is leave your testimonial as a comment and email us your contact details.

Or, if you prefer, email us directly with your quote and contact details and we'll do the rest.

We look forward to hearing from you! In the meantime, Happy Valentine's Day from all at Bioline: The PCR Company.

Introducing Bioline Scholar Monthly

By Bioline: The PCR Company 2 February 2012 No comments

Bioline Scholar

[caption id="attachment_310" align="alignleft" width="147" caption="Bioline Scholar Monthly - Focus on MyTaq™ DNA Polymerase Family"]MyTaq™ DNA Polymerase Family

Keep up-to-date with cutting-edge developments in your particular area of research with Bioline Scholar Monthly, Bioline’s new resource for academic and industry researchers who want to stay ahead of the game.

Our team of scientists will be extracting the best in peer reviewed research and highlighting the different components that make up the diverse ecosystem of life sciences, from genetics to biochemistry, immunology, microbiology, neuroscience, cancer research, ecology, plant sciences and veterinary research.

Learn best practice from laboratory scientists around the world who have already published successfully with Bioline’s range of industry-leading products – designed to accelerate breakthroughs in all major fields of life science.

Thousands of papers cite Bioline products, so if you think we have missed an interesting peer-reviewed publication of yours, featuring any of our products, please get in touch by email, Twitter, Facebook, or leave a comment on the blog. You gain valuable exposure and profile for your group or company and we share your discoveries with the life science world.

Make an impact by connecting with Bioline Scholar Monthly today!


  • Be first to hear about exciting, new, hot-off-the-press papers, novel applications and approaches from the global scientific community
  • Discover highlights of the latest literature from all fields of life sciences featuring Bioline products
  • Post-publication comments/interviews with researchers from the global Bioline community
  • Tried and tested protocols from the literature to accelerate your research
  • Pick up useful scientific hints, tips and tricks
  • Comment and discuss - let us know what you think the latest findings means for your field

Bioline Scholar Monthly: January 2012 Round-Up

The first in our series of Bioline Scholar Monthly compilations focuses on the MyTaq™ DNA Polymerase Family.

MyTaq™ DNA Polymerase

Interesting set of papers for detecting the spread of novel genes from veterinary viruses.

Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011) - Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)

Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011) - Distribution and genetic diversity of porcine hokovirus in wild boars

MyTaq™ Mix

Study of woodland population genetics by 454 shotgun pyrosequencing & PCR. Lead author of the paper, Dr Olivier Lepais, commented:

"We used MyTaq to screen for suitable microsatellite markers in simplex PCR. We found that MyTaq Mix gave great results and was very easy to work with. This ease of use was critical as the primer screening involved a high number of independent PCR mixes!"

Lepais, O. L. & Bacles, C. F. J. Hered. doi: 10.1093/jhered/esr062 (2011) - De Novo Discovery and Multiplexed Amplification of Microsatellite Markers for Black Alder (Alnus glutinosa) and Related Species Using SSR-Enriched Shotgun Pyrosequencing

MyTaq™ Red Mix

Study of microsatellite markers to study the genetic diversity of lobsters in Australia and New Zealand.

Thomas, L. & Bell, J.J. Conserv. Gene. Resources doi: 10.1007/s12686-011-9537-x (2011) - Characterization of polymorphic microsatellite markers for the red rock lobster, Jasus edwardsii (Hutton 1875)

MyTaq™ HS DNA Polymerase

Study on new molecular genetics assay for investigating harmful algal blooms, Sydney Institute of Marine Sciences.

Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011) - sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters

MyTaq™ HS DNA Polymerase

Protocol on preparing ssDNA from PCR products with streptavidin magnetic beads.

Wilson, R. Nucleic Acid Therapeutics doi:10.1089/nat.2011.0322 (2011) - Preparation of Single-Stranded DNA from PCR Products with Streptavidin Magnetic Beads

MyTaq™ HS Mix

Interesting new veterinary parasitology paper from the University of New South Wales, Sydney & Merial, a Sanofi Company.

Šlapeta, J., et al. Vet Parasitol. doi: 10.1016/j.vetpar.2011.03.035 (2011) - The cat flea (Ctenocephalides f. felis) is the dominant flea on domestic dogs and cats in Australian veterinary practices

MyTaq™ HS Mix

Bacteria-triggered histone modification in a fungus showing previously undescribed evidence of Saga/Ada dependent histone acetylation triggered by prokaryotes.

Nützmann, H-W., et al. PNAS 108, 14282-14287 (2011) - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation

MyTaq™ One Step RT-PCR Kit

Neuroscience paper from Department of Cell & Molecular Physiology, Neuroscience Center & Medical School at University of North Carolina.

Buttermore, E.D., et al. J. Neurosci. 31(22), 8013-8024 (2011) - The Cytoskeletal Adaptor Protein Band 4.1B Is Required for the Maintenance of Paranodal Axoglial Septate Junctions in Myelinated Axons

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