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March 2012

Bioline: The PCR Company is 20!

By Bioline: The PCR Company 16 March 2012 No comments

Bioline Celebrates 20 Years of Services to Life Science

How time flies! It's 2012 and this year Bioline: The PCR Company celebrates its 20 year anniversary.

The world has changed rapidly and dramatically since Bioline was first established in 1992 by Marco Calzavara, president of Bioline, but we are still manufacturing and supplying a range of consistently high-quality products to life science researchers and institutions around the world.

From our base in London, UK, we have rapidly expanded to become a leading primary manufacturer and distributor of PCR enzymes and reagents for molecular biology; providing complete solutions to researchers in universities, major research institutions, hospitals, biotech firms, diagnostics and pharmaceutical firms and laboratories globally.

Bioline’s products have been cited thousands of times in leading journals by researchers all over the world and, as part of our 20 year anniversary celebrations, we've started to document some of these publications in our Bioline Scholar Monthly series of blog posts.

When asked about the milestone of two decades of service provision to life science, Marco Calzavara, founder and president of Bioline, commented:

"Our aim at Bioline for the past 20 years has been to develop high-quality and reliable products that really make the difference, delivering consistent results time after time. I am excited and proud to continue Bioline´s great work as a cutting edge molecular biology company, ensuring that we maintain our outstanding reputation as a high-quality and reliable firm."

Richard L. Eberly, Chief Commercial Officer of Meridian Bioscience, Inc., added:

"Congratulations to the Bioline team on its 20th anniversary of successful operations throughout the world. We are enthusiastic about the success of the merger and impressed by the scientific capabilities of Bioline, as well as its well-deserved reputation for innovation and quality. We remain committed to expanding the rapidly growing portfolio of highly specialized molecular biology products from Bioline that enable the development of genomic tests utilized by researchers, clinical diagnostic laboratories, diagnostic test manufacturers and biotechnology companies."

As part of the 20 year celebrations, we are running a monthly Mystery Prize Giveaway for the rest of 2012. Lucky customers will find a golden ticket hiding in their delivery, so keep a close look out when you receive your delivery because you may find one included with your next order!

We'd like all our customers over the years to join in the celebrations and you can do so by visiting the special Bioline 20 Year Anniversary microsite, where we have a timeline showing the major milestones over the last 20 years and an exclusive interview with our president, as well as other Bioline employees. You can also keep up to date with all the latest 20 year anniversary news and updates by following @ThePCRCompany and @BiolineUSA on Twitter.

It just remains to thank all our customers, both old and new alike, for your custom and we look forward to supplying you with more high-quality enzymes and reagents for the next 20 years!

Bioline Scholar Monthly: February 2012 Round-Up

By Bioline: The PCR Company 9 March 2012 No comments

It's time for the second in our series of Bioline Scholar Monthly compilations. This month we're focusing on the many uses of Bioline polymerases in forensic science.

Identification of a report's species is one of the basic analyses in forensics. However, due to the nature of the sampling environment, DNA samples often contain PCR-inhibitory substances which may generate blank or incomplete DNA profiles. The common approach to overcoming PCR inhibition is extensive DNA purification, but this can increase the risk of DNA loss. In some cases, isolation of single cells using laser-capture microdissection can be used, but again this reduces the amount of DNA available.

DNA polymerases that can improve the quality of forensic DNA analysis and efficiently circumvent PCR inhibition, without any additional sample preparation, are therefore advantageous, as are polymerases that result in high yields.

Bioline’s DNA polymerases are very robust and have been carefully designed to overcome these problems. BIO-X-ACT™ Short in particular is specifically designed for difficult/problematic PCR applications that require high processivity and fidelity, applications that would normally fail with other DNA polymerase. MangoTaq™ has also been designed for problematic and ancient DNA, whereas IMMOLASE™ and BIOTAQ™ are high yield for small sample sizes. Together with our new MyTaq™, MyFi™ and RANGER Bioline has polymerases to meet all the high-fidelity requirements of forensic science.

So, without further ado, here's...

Bioline Scholar Monthly: February 2012

BIO-X-ACT™ Short DNA Polymerase

Loop-mediated isothermal amplification (LAMP) is an uncomplicated, quick and relatively inexpensive diagnostic tool. In Barkway and colleagues procedure, BIO-X-ACT Short DNA Polymerase was initially used to successfully verify the LAMP primer pair for Eimeria species specificity using PCR.

Barkway, C. P., et al. BMC Veterinary Research 2011, 7:67 (2011) – Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens

BIO-X-ACT™ Short Mix

In a comparison of nine DNA polymerases, the DNA detection limit was lowest with BIO-X-ACT Short, providing the highest number of improved DNA profiles, using real crime scene saliva samples.

Hedman, J., et al. BioTechniques 47, 951-958 (2009) – Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles

IMMOLASE™ DNA Polymerase

The identification via DNA analysis is reliably and reproducibly possible from well preserved and semi-burnt bones.

Schwark, T., et al Forensic Sci. Int.: Gene. 5(5), 393-399 (2011) – Reliable genetic identification of burnt human remains.

Dried herbarium specimens may be invaluable to understand long-term changes at sites with a history of cyanobacterial blooms.

Metcalf, J.S., et al Harmful Algae 15 47–52 - (2012) – Analysis of microcystins and microcystin genes in 60–170-year-old dried herbarium specimens of cyanobacteria

MangoTaq™ DNA Polymerase

Out of 19 polymerases, the best performance was exhibited by the Mango-Taq DNA polymerase, which was the only polymerase which was able to amplify the ~620 bp amplification product from the 102 year old sample.

Telle, S. & Marco Thines, M. PLoS ONE 3(10):doi:10.1371/journal.pone.0003584 - (2008) – Amplification of cox2 (~620 bp) from 2 mg of Up to 129 Years Old Herbarium Specimens, Comparing 19 Extraction Methods and 15 Polymerases

BIOTAQ™ DNA Polymerase

In Australia and globally, Sarcophagidae flies remain unexploited as indicators of post-mortem interval in forensic investigations. A molecular identification method involving DNA ‘barcoding’ of the mitochondrial COI gene from 16 species of Australian Sarcophagidae was successfully developed. The authors conclude analysis of sarcophagids in forensic entomology should increase and their value as tools in criminal investigations realised.

Meiklejohn, K. A., et al. Int. J. Legal Med. 125(1), 27-32 - (2011) – DNA-based identification of forensically important Australian Sarcophagidae (Diptera)

BIOLASE™ DNA Polymerase

Microsatellite markers were developed for the medicinal plant Tripterygium (Celastraceae) to assess its population structure and to facilitate source tracking of plant materials used for medicinal extracts.

Novy, A. & Jones, K. C. Am. J. Bot. 98(10) e280-e281 (2011) – Characterization of polymorphic microsatellites for Tripterygium (Celastraceae), a monospecific genus of medicinal importance


Lindgren funnel traps baited with aggregation pheromones are effective tools for monitoring flight activity in the red flour beetle (Tribolium castaneum) and lesser grain borer (Rhyzopertha dominica). But the samples are affected by the preservation method.

Stevens, M. M,. et al. J. Stored Products Res. 47(2), 69–75 - (2011) – Maintaining DNA quality in stored-grain beetles caught in Lindgren funnel traps

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