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July 2013

Hands-on Real-Time qPCR Workshop at Birkbeck, sponsored by Bioline

By Bioline: The PCR Company 23 July 2013 No comments

Quantifying nucleic acids by Real-Time qPCR

Hosted by Hosted by qStandard

4th-5th September 2013, Birkbeck, University of London

Register now for a two-day, hands-on workshop sponsored by Bioline: The PCR Company that teaches you the core principles of qPCR and focuses on the practical steps required to design, set-up, validate and analyse Real-Time qPCR assays.

Synthetic biology innovation, building BioBricks with Bioline

By Bioline: The PCR Company 11 July 2013 No comments

The 6th International Meeting on Synthetic Biology (hashtag: #SB6Conf), the world’s foremost synthetic biology (SynBio) meeting, is currently running in London, organised by the BioBricks Foundation, a non-profit organization based in Cambridge, Massachusetts.

BioBricks Foundation SB6.0 Co-Chairs include Professor Paul Freemont and Professor Richard Kitney of Imperial College, who will lead a new £10 million innovation and knowledge centre, to be called SynbiCITE, aimed at providing a bridge between academia and industry and announced at the conference by David Willetts, Minister for Universities and Science.

Imperial College is home to the UK Centre for Synthetic Biology and Innovation a £9m investment aimed at propelling the synthetic biology field forward and promoting SynBio start-ups. International collaboration and networking are important aspects of the meeting, along with poster presentations and ‘lightning talks’ from world leaders in the field of SynBio research.

David Willets said:

"Synthetic biology has huge potential for our economy and society in so many areas, from life sciences to agriculture. But to realise this potential we need to ensure researchers and business work together. This new Innovation and Knowledge Centre will help advance scientific knowledge and turn cutting edge research into commercial success."

Professor Richard Kitney, co-academic of SynbiCITE added:

"Synthetic Biology could be the next ‘industrial revolution’ for the UK, where tiny devices manufactured from cells are used by us to improve many facets of our lives. From producing new, more sustainable fuels to developing devices that can monitor or improve our health, the applications in this field are limitless."

The exciting and emerging field of Synthetic Biology research combines the disciplines of engineering and molecular biology to design and build novel, biologically-based parts, devices, and sensors, as well as the re-engineering of existing, natural biological organisms. Synthetic Biology has the potential to deliver important new applications, from detecting the early onset of disease and improving existing industrial processes, food production, green fuels, and developing therapies to fight harmful bacterial infections or cancers.

Much of the future success of synthetic biology is incumbent upon the development of standardized SynBio components that can be combined in predictable and repeatable ways. The precise approach used when fabricating a BioBrick component depends on the fabrication method (PCR or direct synthesis) as well as the type of part being constructed (a standard part or protein coding sequence).

SyntheticBiology.org maintains a nice introduction to Synthetic Biology as well as a useful how-to guide on the subject of constructing novel BioBrick parts for submission to the Registry of Standard Biological Parts.

Bioline makes it easy to harness the power of new generation enzymes to create parts for BioBricks using PCR. We manufacture and supply a range of high-performance PCR and molecular biology cloning tools, enabling researchers to drive their synthetic biology projects forward. Some of our most popular, most frequently used products for leading synthetic biologists presenting at SB6.0 (1, 2) include the High-Fidelity Velocity DNA Polymerase, MyTaq HS DNA Polymerase for Colony-PCR, Competent Cells, Quick-Stick Ligase for TA Cloning, ISOLATE II Plasmid Mini Kits, and our acclaimed range of SensiMix™ and SensiFAST™ Real-Time PCR kits.

If you've been attending the #SB6Conf in London this week, let us know if your poster cites any Bioline reagents and your synthetic biology research work and achievements could be showcased in a forthcoming SynBio article on the Bioline blog. And if you’re one of the IGEMers attending the conference, don’t forget to check out our Gem of an offer for iGEM Teams!

One final note regarding the future of synthetic biology and the synthetic biologists of the future, you can keep up to date with all the latest news from the iGEM SynBio research teams around the world by following the international iGEM Teams Twitter list maintained by us @ThePCRCompany.

Citations

1. Giuraniuc CV, MacPherson M, Saka Y, et al. (2013). Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae. PLoS ONE 8(5): e64419. doi:10.1371/journal.pone.0064419

2. Ali H, Ries MI, Nijland JG, Lankhorst PP, Hankemeier T, et al. (2013). A Branched Biosynthetic Pathway Is Involved in Production of Roquefortine and Related Compounds in Penicillium chrysogenum. PLoS ONE 8(6): e65328. doi:10.1371/journal.pone.0065328

New Biotechnology Partnership Promises to Benefit Genomics Scientists in Ireland's Leading Research Centres

By Bioline: The PCR Company 8 July 2013 No comments

Bioline and MSC Partnership Bioline/MSC Irish Partnership

Bioline: The PCR Company and Medical Supply Company Ltd (MSC) of Dublin, Ireland are pleased to announce an exclusive partnership deal to deliver high-quality, high-performance Bioline reagents to hospitals, universities, technological institutes, industrial and pharmaceutical companies throughout Ireland.

From 9th July 2013, MSC becomes the exclusive distributor in the Republic of Ireland and Northern Ireland for the complete range of high-performance Bioline reagents and kits, with a focus on PCR and Real-Time PCR products.  Eimear Scully, Managing Director of MSC commented:

Bioline has an excellent reputation for quality products and customer value, which closely matches our own values. MSC is committed to delivering the best solutions to meet the changing needs of our customers in each area we serve and we believe Bioline offers these quality solutions for PCR and Real-Time PCR."

Marco Calzavara, President of Bioline added:

"We are very excited with the appointment of MSC as this significantly expands our presence in Ireland through the expertise of MSC's team of highly qualified application specialists. We believe the partnership will enable scientists located in the leading Irish research centres to realize the benefits of our widely used PCR reagents."

From today MSC customers will have access to high-performance reagents and kits such as the proven SensiFAST™ Real-Time reagents, which offer the greatest sensitivity and fastest reaction times in Real-Time applications. MSC customers will also benefit from direct access to the highly-praised technical support services available from Bioline laboratory scientists.

Richard L. Eberly, President of Meridian Life Science, Inc., said:

We are delighted with the appointment of Medical Supply Company as our exclusive distributor for Bioline reagents and kits in Ireland. We chose MSC because of their commitment to customer service and technical expertise in the PCR area and we are confident this will result in delivering a world class service to our customers in Ireland."

For more information, please contact the Bioline office in London or:

Medical Supply Company Ltd.

Tel: +353 (0)1 822 4222

Fax: +353 (0)1 822 4100

Web: www.medical-supply.ie

Email: info@medical-supply.ie

Bioline Launches the MyTaq™ Extract-PCR Kit — Single Tube Extraction of PCR-Ready DNA from Mammalian Tissue

By Bioline: The PCR Company 4 July 2013 No comments

Bioline: The PCR Company, a wholly-owned subsidiary of Meridian Bioscience, Inc. (NASDAQ:VIVO), is proud to announce the worldwide release of the MyTaq™ Extract-PCR Kit.

Powered by the high-performance MyTaq™ DNA Polymerase, the MyTaq™ Extract-PCR Kit offers a quick and easy alternative for the extraction and amplification of DNA from a variety of mammalian tissue types.

Researchers using the MyTaq™ Extract-PCR Kit can replace complicated, time-consuming extraction methods with a simple 15 minute, single tube, protocol; making it perfect for high-throughput applications, such as mouse genotyping and sequencing.

Marco Calzavara, President of Bioline commented:

"With the MyTaq™ Extract-PCR Kit researchers can now quickly achieve the purity and yield necessary for high-performance PCR and Real-Time PCR experiments from solid tissues, such as mouse tail or mouse ear. This makes the MyTaq™ Extract-PCR Kit the perfect addition to our widely used PCR reagents."

Richard L. Eberly, President of Meridian Life Science, Inc., stated:

"We are delighted to release the MyTaq™ Extract-PCR Kit, another product in the rapidly expanding portfolio of highly specialized molecular biology reagents from Bioline. We are committed to our life science customers and to bringing innovation and quality products; such as the family of MyTaq™ products to the research laboratory, clinical diagnostic laboratories, and biotechnology companies."

Learn more and order the new MyTaq™ Extract-PCR Kit from www.bioline.com.

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