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Agarose gel electrophoresis is a common molecular biology technique used to separate DNA or RNA molecules by size. This is achieved by denaturing and applying a negative charge to our nucleic acid sample. The samples are then run through an agarose matrix with an electric field (Electrophoresis). Shorter molecules move faster and thus migrate further and in the gel over a given time period. Many factors are involved with regards to the final visualization step and achieving high resolution of the resulting bands on the agarose gel.
Tips to help improve resolution include: - Running the gel at a lower voltage for a longer period of time - Using a wider/thinner gel comb - Loading less DNA into each well
MangoTaq comes with a coloured reaction buffer that contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel.
We have created a #qPCR Infographic that discusses the origins of qPCR, applications, #NGS, current technology and more. To get a copy to put in your lab, or to view on your tablet and smartphone then email us directly with #qPCRINFO in the subject line at >> email@example.com
#qPCR #PCR #Infographic #NGS #Mic
"I am a Business Development Manager for Bioline, responsible for academic and industrial accounts in London, Oxford, Cardiff and Swansea. It’s delightful to experience first-hand in meetings the differing dynamics the regions and institutions have; whilst some will focus on the technical aspects of our specific product lines and others will focus on what we offer in Custom Solutions
I have been with Bioline for almost five years and it has been great to see the company grow in so many ways. To have been a part of so many product launches and knowing that it has been my friends in R&D responsible for the quality, makes me that much more passionate and proud of being a part of the team here. Receiving great feedback from my customers only amplifies this passion further. There is a great family feel here and living close to the office means I get enjoy coming in and catching up with good friends.
In my spare time, I am Chairman and loosehead prop for Ickenham Rugby Club. Responsible for 60 players and have a great committee to handle the fixtures, fitness, finances, marketing and social aspects of the club. The day after a game, I am usually recovering with some knitting projects."
Manvir Tiwana, Business Development Manager
Thank you, Sharon Pok from the Australian National University Medical School, for a great review on our ISOLATE II RNA Mini Kit.
"I recently used the ISOLATE II RNA Mini Kit. I had some cultured cells which are derived after FACS, which are not very many, to extract RNA from. Because I had a small starting quantity (<1E6 cells), I wasn’t expecting an extremely high yield. However, I attained a relatively good yield from that small amount of starting sample. What also impressed me was the 260/280 and 260/230 ratios. I have been having trouble getting a good 260/230 ratio lately, probably because of residual guanidine not being washed off properly. With the kit, both ratios were well above 2.0. Waiting to run the samples on the Bioanalyzer to obtain RIN values."
Click here to view Sharon's Results >> ISOLATE II RNA Mini Kit
Click here to learn more about our >> ISOLATE II Nucleic Acid Isolation family
Thank you, Joey Liu from Ohio State University, for a great review on our SensiFAST™ SYBR® No-ROX Kit.
"The performance of the SensiFAST™ SYBR® No-ROX Kit is robust. We have used it with ABI 7900 and Roche LightCycler 480 for gene expression for a while. The studies include a variety of human and mouse primary cells and cell lines. We are very happy with the kit because of its sensitivity and repeatability and will continue to go with it."
Click here to learn more about >> SensiFAST™ SYBR® No-ROX Kit
Thank you, Kwan Tzu Nin from The Institute for Marine and Antarctic Studies, for a great review on our MyTaq™ Extract-PCR Kit.
"MyTaq™ Extract-PCR Kit was effective and efficient for high throughput genotyping using as little as 1 mg of fish fin clip samples"
Click here to learn more about >> MyTaq™ Extract-PCR Kit
Thank you, Kelly Condon from James Cook University, for a great review on our Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes.
"Having used the Bioline Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes in our research for the past 2-3 years, I have no hesitation in recommending them. Besides offering great economy in purchase costs, the reagents perform with great accuracy, sensitivity and consistency in our assays which gives us great reproducibility in our results."