Real-Time PCRRSS Feed
Thank you, Kelly Condon from James Cook University, for a great review on our Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes.
"Having used the Bioline Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes in our research for the past 2-3 years, I have no hesitation in recommending them. Besides offering great economy in purchase costs, the reagents perform with great accuracy, sensitivity and consistency in our assays which gives us great reproducibility in our results."
Thank you, Benson Lindsey from The Ohio State University, for a great review on our MyTaq Plant Direct PCR Kit.
"We tested the MyTaq Plant Direct PCR Kit in our Arabidopsis genotyping workflow. It saved us a lot of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20 oC and reused multiple times over several weeks, with no discernible loss of quality. We also were able to use MyTaq Plant Direct PCR Kit to 'rescue' genomic DNA, resulting from poor quality isolations that would not amplify with other polymerases.
MyTaq Plant Direct PCR Kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50 mL reaction, only the MyTaq Plant Direct PCR Kit was able to amplify directly from leaf tissue in a 25 mL reaction."
Thank you Dr Aparna Jayachandran from Greenslopes Private Hospital, for a great review on our ISOLATE II RNA Mini Kit.
"We have been utilizing ISOLATE II RNA Mini Kit from Bioline and have found that the RNA yield, concentrations, and purity have been consistently good. From 40,000 liver cancer (mouse and human) cells we are able to get 200-600ng/ul yield of RNA."
Click the link to learn more about >> ISOLATE II RNA Mini Kit
Thank you Rebecca Traub from University of Melbourne, for a great review on our ISOLATE Fecal DNA Kit
"Aim: To compare the sensitivity of two commercial faecal DNA extraction kits for the detection and quantification of human hookworms (Ancylostoma ceylanicum, Ancylostoma duodenale and Necator americanus) eggs in faeces.
Methods: Ten individual human faecal samples positive for hookworm eggs were each extracted with two commercial kits, the Powersoil DNA Isolation Kit (MoBio) and the ISOLATE Fecal DNA Kit (Bioline) according to manufacturer's instructions. DNA was eluted to 100 ul for each.
DNA was subjected to multiplex Taq-man based multiplex qPCR (unpublished) targeting the internal transcribed spacer regions of the three human hookworms and an internal control (EHV4).
Results: Both kits performed equivocally in positively detecting at least one species of hookworm in all ten samples. The ISOLATE Fecal DNA Kit produced on average 0.5 Ct lower threshold cycles for samples compared to the Powersoil DNA Isolation Kit. The time taken to extract 10 samples was significantly less for the Bioline product.
Conclusion: The Powersoil DNA Isolation Kit and the ISOLATE Fecal DNA Kit are comparable in terms of sensitivity for detecting and quantifying DNA of hookworms from human faecal samples. The Bioline kit was less labour intensive compared to the Mo Bio kit and is more competitively priced."
Click the link to view the data >> Hookworm Multiplex Kit Data
Click the link to learn more about >> ISOLATE Fecal DNA Kit
Thank you Jonathan Ferrand from Hudson Institute of Medical Research, VIC Australia, for a great review on our MyTaq™ Red Mix
"We have been using the MyTaq™ Red Mix for the past two years in our routine PCRs for mice genotyping, cloning/amplicon validation and standard curve preparation for our RTqPCR. This product is very convenient with gel loading buffer included, and in our hands work as well as competitor products with as low as 5ul of Mix per reaction (10ul final volume). The competitive pricing makes it a very attractive alternative for routine PCRs."
Click the link to learn more about >> MyTaq™ Red Mix
Initially, the RT step should be performed as specified in the supplier protocol. However, the length and the temperature of the RT step can be optimized to increase the efficiency of the reverse transcriptase. The reverse transcriptase should be tested across a range of RNA concentrations to ensure assay linearity.