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  • 16 August 2016

    Thank you Rebecca Traub from University of Melbourne, for a great review on our ISOLATE Fecal DNA Kit

    "Aim: To compare the sensitivity of two commercial faecal DNA extraction kits for the detection and quantification of human hookworms (Ancylostoma ceylanicum, Ancylostoma duodenale and Necator americanus) eggs in faeces.

    Methods: Ten individual human faecal samples positive for hookworm eggs were each extracted with two commercial kits, the Powersoil DNA Isolation Kit (MoBio) and the ISOLATE Fecal DNA Kit (Bioline) according to manufacturer's instructions. DNA was eluted to 100 ul for each.

    DNA was subjected to multiplex Taq-man based multiplex qPCR (unpublished) targeting the internal transcribed spacer regions of the three human hookworms and an internal control (EHV4).

    Results: Both kits performed equivocally in positively detecting at least one species of hookworm in all ten samples. The ISOLATE Fecal DNA Kit produced on average 0.5 Ct lower threshold cycles for samples compared to the Powersoil DNA Isolation Kit. The time taken to extract 10 samples was significantly less for the Bioline product.

    Conclusion: The Powersoil DNA Isolation Kit and the ISOLATE Fecal DNA Kit are comparable in terms of sensitivity for detecting and quantifying DNA of hookworms from human faecal samples. The Bioline kit was less labour intensive compared to the Mo Bio kit and is more competitively priced."


    Click the link to view the data >> Hookworm Multiplex Kit Data

    Click the link to learn more about >> ISOLATE Fecal DNA Kit

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