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By Bioline: The PCR Company 2 February 2012 Leave a comment Go to comments

Bioline Scholar

[caption id="attachment_310" align="alignleft" width="147" caption="Bioline Scholar Monthly - Focus on MyTaq™ DNA Polymerase Family"]MyTaq™ DNA Polymerase Family

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Bioline Scholar Monthly: January 2012 Round-Up

The first in our series of Bioline Scholar Monthly compilations focuses on the MyTaq™ DNA Polymerase Family.

MyTaq™ DNA Polymerase

Interesting set of papers for detecting the spread of novel genes from veterinary viruses.

Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011) - Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa)

Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011) - Distribution and genetic diversity of porcine hokovirus in wild boars

MyTaq™ Mix

Study of woodland population genetics by 454 shotgun pyrosequencing & PCR. Lead author of the paper, Dr Olivier Lepais, commented:

"We used MyTaq to screen for suitable microsatellite markers in simplex PCR. We found that MyTaq Mix gave great results and was very easy to work with. This ease of use was critical as the primer screening involved a high number of independent PCR mixes!"

Lepais, O. L. & Bacles, C. F. J. Hered. doi: 10.1093/jhered/esr062 (2011) - De Novo Discovery and Multiplexed Amplification of Microsatellite Markers for Black Alder (Alnus glutinosa) and Related Species Using SSR-Enriched Shotgun Pyrosequencing

MyTaq™ Red Mix

Study of microsatellite markers to study the genetic diversity of lobsters in Australia and New Zealand.

Thomas, L. & Bell, J.J. Conserv. Gene. Resources doi: 10.1007/s12686-011-9537-x (2011) - Characterization of polymorphic microsatellite markers for the red rock lobster, Jasus edwardsii (Hutton 1875)

MyTaq™ HS DNA Polymerase

Study on new molecular genetics assay for investigating harmful algal blooms, Sydney Institute of Marine Sciences.

Murray, S.A., et al. Appl. Envir. Microbiol. 77, 7050-7057(2011) - sxtA-Based Quantitative Molecular Assay To Identify Saxitoxin-Producing Harmful Algal Blooms in Marine Waters

MyTaq™ HS DNA Polymerase

Protocol on preparing ssDNA from PCR products with streptavidin magnetic beads.

Wilson, R. Nucleic Acid Therapeutics doi:10.1089/nat.2011.0322 (2011) - Preparation of Single-Stranded DNA from PCR Products with Streptavidin Magnetic Beads

MyTaq™ HS Mix

Interesting new veterinary parasitology paper from the University of New South Wales, Sydney & Merial, a Sanofi Company.

Šlapeta, J., et al. Vet Parasitol. doi: 10.1016/j.vetpar.2011.03.035 (2011) - The cat flea (Ctenocephalides f. felis) is the dominant flea on domestic dogs and cats in Australian veterinary practices

MyTaq™ HS Mix

Bacteria-triggered histone modification in a fungus showing previously undescribed evidence of Saga/Ada dependent histone acetylation triggered by prokaryotes.

Nützmann, H-W., et al. PNAS 108, 14282-14287 (2011) - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation

MyTaq™ One Step RT-PCR Kit

Neuroscience paper from Department of Cell & Molecular Physiology, Neuroscience Center & Medical School at University of North Carolina.

Buttermore, E.D., et al. J. Neurosci. 31(22), 8013-8024 (2011) - The Cytoskeletal Adaptor Protein Band 4.1B Is Required for the Maintenance of Paranodal Axoglial Septate Junctions in Myelinated Axons

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