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Thank you, Kelly Condon from James Cook University, for a great review on our Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes.
"Having used the Bioline Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes in our research for the past 2-3 years, I have no hesitation in recommending them. Besides offering great economy in purchase costs, the reagents perform with great accuracy, sensitivity and consistency in our assays which gives us great reproducibility in our results."
Thank you, Benson Lindsey from The Ohio State University, for a great review on our MyTaq Plant Direct PCR Kit.
"We tested the MyTaq Plant Direct PCR Kit in our Arabidopsis genotyping workflow. It saved us a lot of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20 oC and reused multiple times over several weeks, with no discernible loss of quality. We also were able to use MyTaq Plant Direct PCR Kit to 'rescue' genomic DNA, resulting from poor quality isolations that would not amplify with other polymerases.
MyTaq Plant Direct PCR Kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50 mL reaction, only the MyTaq Plant Direct PCR Kit was able to amplify directly from leaf tissue in a 25 mL reaction."
"I am the U.S. Mid-Atlantic Senior Account Manager for Bioline. I’m the person people see when they want faster PCR or want advice about improving a protocol. You may see me at a conference, your lab, a vendor show, or just in your institute’s coffee shop. Say hello!
As a scientist with 10 years of research experience, focusing on drug development, (Candida albicans, biophysics, and Giardia lamblia) I use my scientific background every day to understand what you are doing in the lab and providing you with the right Bioline reagent to give you the best yield. I was actually a Bioline customer before I joined the team. I’ve personally experienced how Bioline works closely with their customers; doing everything they can to make sure I gained the best results from my research. This is all thanks to my rep at the time, who is now my manager!
The challenges of this job are rewarding; whether it’s learning a new protocol, solving a need for a custom product, or seeing a client’s results. But most of all it is the relationships that are built and the achievements that I see that are most exciting!
In my spare time, I’m an artist and entrepreneur. I’m fascinated by alternative process photography with the use of non-traditional chemistry; there’s something wonderful about watching an image develop on a piece of paper with handmade emulsion in a darkroom."
Jesse Cohen, Senior Account Manager
Thank you Dr Aparna Jayachandran from Greenslopes Private Hospital, for a great review on our ISOLATE II RNA Mini Kit.
"We have been utilizing ISOLATE II RNA Mini Kit from Bioline and have found that the RNA yield, concentrations, and purity have been consistently good. From 40,000 liver cancer (mouse and human) cells we are able to get 200-600ng/ul yield of RNA."
Click the link to learn more about >> ISOLATE II RNA Mini Kit
Bioline GmbH’s 15th Year Anniversary
“Bioline and the team in Germany have built a first-class, ISO-13485-compliant, world-class manufacturing operation in Luckenwalde, Germany that enables our global life science business to continue to grow and penetrate new markets. It is a great privilege to present to our customers around the world that we manufacture all of our own high-quality enzymes, dNTP’s, and master mixes at Bioline GmbH.” - Richard L. Eberly, MBI
Thank you Rebecca Traub from University of Melbourne, for a great review on our ISOLATE Fecal DNA Kit
"Aim: To compare the sensitivity of two commercial faecal DNA extraction kits for the detection and quantification of human hookworms (Ancylostoma ceylanicum, Ancylostoma duodenale and Necator americanus) eggs in faeces.
Methods: Ten individual human faecal samples positive for hookworm eggs were each extracted with two commercial kits, the Powersoil DNA Isolation Kit (MoBio) and the ISOLATE Fecal DNA Kit (Bioline) according to manufacturer's instructions. DNA was eluted to 100 ul for each.
DNA was subjected to multiplex Taq-man based multiplex qPCR (unpublished) targeting the internal transcribed spacer regions of the three human hookworms and an internal control (EHV4).
Results: Both kits performed equivocally in positively detecting at least one species of hookworm in all ten samples. The ISOLATE Fecal DNA Kit produced on average 0.5 Ct lower threshold cycles for samples compared to the Powersoil DNA Isolation Kit. The time taken to extract 10 samples was significantly less for the Bioline product.
Conclusion: The Powersoil DNA Isolation Kit and the ISOLATE Fecal DNA Kit are comparable in terms of sensitivity for detecting and quantifying DNA of hookworms from human faecal samples. The Bioline kit was less labour intensive compared to the Mo Bio kit and is more competitively priced."
Click the link to view the data >> Hookworm Multiplex Kit Data
Click the link to learn more about >> ISOLATE Fecal DNA Kit