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It’s that time of year again!!!! We are proud to announce Bioline is supporting iGEM teams in 2015. Our already competitive pricing drops 40% for the young and enthusiastic iGEM researchers in the United Kingdom.
‘Sponsorship is very difficult, particularly for small projects such as ours. Bioline are very kindly sponsoring us. A big thank you to them.’ - York iGEM 2014 Team
Popular tools used by successful iGEM teams around the world include:
- Bioline Competent Cells and Cloning Reagents - Transform plasmids from the iGEM kit plates with extremely efficient alpha-select silver or gold efficiency cells.
- MyTaq™ HS - Use MyTaq HS to amplify DNA from your minipreps in as little as 30 minutes, or amplify directly from your colonies! See the MyTaq colony PCR application note or this SOP written by the Danish iGEM2013 team.
- ISOLATE II Plasmid Mini Kit - Pure and very high DNA yields from your transformed colonies.
- VELOCITY DNA Polymerase - Perfect for all your high-fidelity applications.
- HyperLadder™ DNA Markers - Very popular Bioline range of markers for DNA sizing, as used by Craig Venter's research team.
- Real-time PCR Kits - Trusted SensiFAST™ kits for gene expression analysis.
- Core Reagents - High quality buffers, agarose and other essentials.
Get in touch with your local UK Bioline Account Manager to enquire about an iGEM offer pricing plan.
Then, why not let your fellow iGEM researchers know about this great offer using the sharing links below? Also feel free to Tweet or email us if you have any further questions www.bioline.com/social We look forward to hearing from you soon and we wish all iGEM2015 entrants every success in this year's competition!
Terms and Conditions
This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on September 30, 2015. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia.
Bioline is proud once again to offer its support to teams participating in the 2014 round of the annual iGEM competition. Teams supported by Bioline in previous years have won gold medals and advanced to the finals of the iGEM World Championships.
For a limited time only, all iGEM teams in the United Kingdom can take advantage of up to 40% off already highly-competitive Bioline list prices across our entire product range.
The Must-Have Bioline Toolbox for iGEM Researchers
Popular tools used by successful iGEM teams around the world include:
- Bioline Competent Cells and Cloning Reagents — Transform plasmids from the iGEM kit plates with Bioline’s extremely efficient alpha-select silver or gold efficiency cells.
We used bioline cells but didn’t follow the protocols because the cells were more competent so didn’t need to go through heat shock.— Boston iGEM 2012 Team
- MyTaq™ HS — Use MyTaq HS to amplify DNA from your minipreps in as little as 30 minutes, or amplify directly from your colonies! See the MyTaq colony PCR application note or this SOP written by the Danish iGEM2013 team.
- ISOLATE II Plasmid Mini Kit — Pure and very high DNA yields from your transformed colonies.
- VELOCITY DNA Polymerase — Perfect for all your high-fidelity applications.
- HyperLadder™ DNA Markers — Our hugely popular range of markers for DNA sizing, as used by Craig Venter's research team!
- Real-time PCR Kits — Tried and trusted SensiFAST kits for gene expression analysis.
- Core Reagents — High quality buffers, agarose and other essentials.
All Bioline products are backed by our friendly and helpful UK based technical support staff, should you need it!
To request full details of our Gem of an iGEM Offer pricing plan, please contact your local Bioline UK account manager using our Rep Finder tool. Then, why not let your fellow iGem researchers know about this great offer using the sharing links below. Also feel free to Tweet or email us if you have any further questions
We look forward to hearing from you soon and we wish all iGEM2014 entrants every success in this year's competition!
PS: You can also read about Bioline’s Synthetic Biology industry partnership with Liverpool Life Sciences UTC, the first school in the United Kingdom specialising in Science and Health Care for 14 to 19 year olds.
This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on October 31, 2014. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia. Bioline products are also available through our carefully selected distributor partners.
An alternative way for RNAi libraries to modify the mammalian genome on a large-scale may lie in the use of genome editing technologies such as the use of targeted endonucleases. Such a technique includes Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly-interspaced, short palindromic repeats/CRISPR-associated endonuclease cas9 (CRISPR/Cas9). CRISPR is a Cas9 endonuclease–based method for sequence-specific genome modification. It can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 20-nt targeting sequence within its guide RNA.
These technologies have proven highly useful for gene editing genomes of various species and the speed at which CRISPR techniques have been exploited has been incredibly rapid.
In January 2013 researchers at MIT, the Broad Institute, and Rockerfeller University first reported the new technique based on the CRISPR/Cas9 system, for precisely altering the genomes of living cells through gene addition or deletion. Researchers say the technology could offer an easy-to-use and more cost-effective way to engineer organisms that produce biofuels; to design animal models to study human disease; or to develop new therapies, among other potential applications.
Bioline reagents have been used to augment the workflow in these exciting gene editing technologies to elucidate CRISPR systems, including our SensiFAST™ range of Real-Time PCR Kits, as well as our performance PCR polymerases.
In the first examples of genes that mediate the inhibition of a CRISPR/Cas system, researchers from the University of Toronto identified five distinct ‘anti-CRISPR’ genes in the genomes of bacteriophages infecting Pseudomonas aeruginosa.
Bondy-Denomy, J et al., (2012). Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature 493, 429-432.
Researchers from Minnesota University reported precise, high-frequency editing of 15 genes in pig, goat, and cattle genomes using CRISPR/Cas and TALENs technology. RFLP analysis of colonies were analysed by PCR using MyTaq Red Mix.
Wenfang, T.W. et al., (2013). Efficient nonmeiotic allele introgression in livestock using custom endonucleases. PNAS 110(41), 16526-16531.
To learn more about performance PCR reagents from Bioline, just visit www.bioline.com
Bioline Launches the MyTaq™ Extract-PCR Kit — Single Tube Extraction of PCR-Ready DNA from Mammalian Tissue
Powered by the high-performance MyTaq™ DNA Polymerase, the MyTaq™ Extract-PCR Kit offers a quick and easy alternative for the extraction and amplification of DNA from a variety of mammalian tissue types.
Researchers using the MyTaq™ Extract-PCR Kit can replace complicated, time-consuming extraction methods with a simple 15 minute, single tube, protocol; making it perfect for high-throughput applications, such as mouse genotyping and sequencing.
Marco Calzavara, President of Bioline commented:
"With the MyTaq™ Extract-PCR Kit researchers can now quickly achieve the purity and yield necessary for high-performance PCR and Real-Time PCR experiments from solid tissues, such as mouse tail or mouse ear. This makes the MyTaq™ Extract-PCR Kit the perfect addition to our widely used PCR reagents."
Richard L. Eberly, President of Meridian Life Science, Inc., stated:
"We are delighted to release the MyTaq™ Extract-PCR Kit, another product in the rapidly expanding portfolio of highly specialized molecular biology reagents from Bioline. We are committed to our life science customers and to bringing innovation and quality products; such as the family of MyTaq™ products to the research laboratory, clinical diagnostic laboratories, and biotechnology companies."
Learn more and order the new MyTaq™ Extract-PCR Kit from www.bioline.com.
Australia Day traditionally marks the anniversary in 1788, when Captain Arthur Philip and the First Fleet arrived at Port Jackson, now part of Sydney, New South Wales, to establish the first European settlement. The holiday is an opportunity for Australians to celebrate the founding of the country and its culture.
Bioline is also privileged to call Sydney home. For 10 years, Bioline Australia has been proud to be a primary manufacturer and supplier of best-in-class molecular biology tools to the leading research universities and institutes in Australia. Bioline's new R&D facility in the Australian Technology Park in Sydney was officially opened by the Hon. Verity Firth MP (Minister for Science and Medical Research) in November 2007. For those interested, photos from the launch are available here.
We thought the Australia Day celebrations might be a good time to highlight some of the scientific achievements of our antipodean cousins, so this month's Bioline Scholar features a selection of interesting, recently published papers from scientists 'down under'.
Blyton M.D. et al. (2013). High temporal variability in commensal Escherichia coli strain communities of an herbivorous marsupial. Environ. Microbiol. doi: 10.1111/1462-2920.12088
In this study from The Australian National University in Canberra, commensal E. coli strains of mountain brushtail possums were quantified at both the host population level and within individuals. E. coli strains were identified using rep-PCR profiling and quadruplex PCR. A high level of temporal variability was found.
Luter, H.M. et al. (2012). Thermal and sedimentation stress are unlikely causes of brown spot syndrome in the coral reef sponge, Ianthella basta. PLoS ONE 7(6): e39779.
Brown spot lesions currently affect a large population of I. basta, an ecologically important sponge species on the Great Barrier Reef. Researchers from the Australian Institute of Marine Science at James Cook University found changes in the microbial community of I. basta to be stable. Thermal and sedimentation stress were not likely to be responsible for the syndrome.
Kelly, R.D. et al. (2013). Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells. Stem Cells doi: 10.1002/stem.1313
In this paper from the Monash Institute of Medical Research in Melbourne, the effects of different mitochondrial DNA haplotypes on differentiation and development were studied using embryonic stem cell lines. In conclusion, mitochondrial DNA haplotypes play a pivotal role in the process of differentiation and mediate cell fate.
Roberts, T. et al. (2013). Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. Vet. Parasit. doi: 10.1016/j.vetpar.2013.01.011
Researchers from St Vincent's Hospital and the University of Technology, Sydney identified nine different genetic subtypes of Blastocytis, a genus of Protozoan parasite, in different animal species in NSW. Blastocystis was identified for the first-time from the eastern grey kangaroo, red kangaroo, wallaroo, snow leopard and ostrich. Further investigation into the genetic diversity of Blastocystis may help identify the zoonotic potential of Blastocystis.
Covelloa, J.M. et al. (2013). Isolation of RAG-1 and IgM transcripts from the striped trumpeter (Latris lineata), and their expression as markers for development of the adaptive immune response. Fish Shellfish Immun. doi: 10.1016/j.fsi.2012.12.015
In this work from the National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, mRNA sequences from the RAG-1 and IgM heavy chain were analysed from the striped trumpeter (Latris lineata). The expression of the two genes was assessed throughout the early developmental stages of striped trumpeter larvae and used as markers to follow the ontogeny of the adaptive immune response.
That's it for this edition of Bioline Scholar! If you have published research using Bioline products, then get in touch, tell us and your paper could make it into a future edition of Bioline Scholar.
We caught Fides Lay in the lab on the day of a basketball game between UCLA and crosstown arch-rival USC. As a former UCLA undergrad now working on her PhD at USC, she never wants to miss a game between the two. Unfortunately, the long hours in the lab don’t always allow her to be home in time to watch. At least, that’s how it used to be for her. Thanks to Bioline’s MyTaq HS Mix, now she doesn’t miss a game.
In Peter Jones’ lab at USC, Fides is part of a team studying the epigenetic regulation of cancer. And as anyone who ever worked on epigenetics knows, you need to be a master of PCR to get any results at all. When you need to amplify bisulfite converted DNA to measure methylation status, you work with small samples that often have been digested with several enzymes, with DNA that is fragmented and damaged.
For the longest time the lab amplified bisulfite converted DNA with a tedious protocol; tedious because of the long set-up with using Taq from a previous supplier, DMSO, and other components added one at a time, and extremely slow cycling with a PCR protocol that was 7-11 hours long.
Anything could go wrong at any time, and the result was impossible to predict. Simply using a different thermocycler with a different ramping speed could cause the reaction to fail. And once you found out, it would be too late to fix because every new attempt takes a whole other day. Since the lab usually clones and sequences the PCR fragments, there was always still the risk that no clones would have inserts. Hard to predict, and that meant starting over.
All that changed when Fides first tried Bioline’s MyTaq HS Mix, an easy, all-in-one mix that contains the enzyme, dNTPs, buffer and all optimizers. There’s no need to add any DMSO, it works right away on almost all templates. And it's fast! Reactions are done in less than two hours, even on bisulfite converted DNA, with highly consistent results, and always with nice bands. The PCR products are much easier to clone, and on the rare occasion that something does go wrong, there’s still time to redo the experiment AND get the samples off to pyrosequencing the same day.
Now Fides has time to run multiple experiments and redo anything that goes wrong, all in time to get home, kick up her feet and watch the game.