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  • qPCR Infographic

    16 February 2017
    qPCR Inforgraphic

    We have created a #qPCR Infographic that discusses the origins of qPCR, applications, #NGS, current technology and more. To get a copy to put in your lab, or to view on your tablet and smartphone then email us directly with #qPCRINFO in the subject line at >> social@bioline.com

    #qPCR #PCR #Infographic #NGS #Mic

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    Bioline SensiFAST™ cDNA Synthesis Kit

    9 September 2016



    The best reward for us is hearing achievements through Bioline products, Brendan. S from University of Arizona: Arizona Biomedical Collaborative reviews Bioline SensiFAST™ cDNA Synthesis Kit”



    Posted in: BiolinePCRReal-Time PCR Tags: testimonialBiolineAccuzymePCRqPCREpik

    MIC qPCR Official Sponsor James Winslow Racing at 24 Heures Le Mans

    21 June 2016



    MIC qPCR by Bio Molecular Systems is the official Sponsor of James Winslow Racing at 24 Heures du Mans.

    Click To Learn More About MIC Here
    #qPCRr #PCR #biotechnologies #lab #LEMANS24 #TakeTheMIC

    Posted in: Bioline Tags: qPCRPCRbiotechnologieslabLEMANS24TakeTheMIC

    MIC Testimonial

    20 June 2016



    Dr. Christoph Zimmer from the University of Exeter has given a #Testimonial about MIC. Thank you! We look forward to hearing more about the great work performed with MIC. #TakeTheMIC

    Posted in: Bioline Tags: MICtestimonialMICqPCR

    Hints, Tips & Tricks - Annealing Temperature

    18 February 2016

    Test a range of annealing temperatures. Depending on the qPCR results, the annealing temperature should be increased or decreased in 2-3oC increments. This can be done in a single experiment using a thermal gradient. Alternatively, a range of annealing temperatures should be tested using multiple qPCR experiments.

    Posted in: BiolinePCR Tags: qPCRAnnealing Temperature

    Seeding Campaign Share Your Review

    22 January 2016

    Thank you B.Dhungel, from Gallipoli Medical Research Foundation (GMRF), Greenslopes Private Hospital, University of Queensland for such a great review.

    I am really impressed by the reproduciblity of the Bioline Isolate II RNA minikit, the cDNA sysnthesis and the Sybr-Lo Rox mix in qPCR. We consistently get high concentrations of pure RNA with 260/280 reading 2 when measured with the nanodrop.

    We have seen that the cDNA synthesis kit from Bioline is able to synthesize high quality cDNA even from low starting RNA concentrations when analyzing the qPCR results.

    Hints, Tips and Tricks

    14 January 2016

    Working with a new qPCR target

    When looking at a new target of unknown expression level, the amount of RNA required to detect the target of interest depends on the abundance of the target in each sample. For high-copy-number transcripts you may be able to detect in as little as 10 pg, while for low-copy-number transcripts you may require more than 100 ng.

    With all new targets (new sets of primers or new qPCR kits), a 10 fold serial dilution should always be run, to validate both the slope and the limit of detection (LOD) (the highest Ct value observed for a truly positive sample, as verified by melt-curve analysis). Only a single peak, which represents the specific PCR product, should be observed on the melt curve, the presence of other peaks indicates the presence of primer–dimers and/or nonspecific PCR products which can contribute to a stronger fluorescence signal and an earlier Ct, but will not be as sensitive.

    Posted in: BiolineLab Hints & TipsPCR Tags: PCRqPCR