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  • Simon Baker, Bioline Global R&D Director Discusses the Future of R&D

    12 January 2017



    Simon Baker PhD FRSB, is our Global R&D Director. In this video he takes the time to discusses the growth of R&D; our bigger team, new products, and strategies.

    To watch our Values video,click here

    Posted in: Bioline Tags: VideoValuesMissionStrategyR&D

    Dr Simon Baker as a BBSRC Committee Member

    23 January 2016

    Our very own Dr Simon Baker, Senior Director of R&D discussing the value of his role as BBSRC committee member

    Check it out here...

    #BBSRC

    Posted in: Bioline Tags: BBSRCSimon BakerR&D

    R&D Technical Support Success Story

    23 December 2015

    Our R&D team is always there to help directly with any problems and queries. We take pride in our teams capabilities to produce fantastic results for our customers.

    Dear Ana,

    The Ct values are close to the negative because we were extracting the DNA in an inappropriately way. It is our fault. It is not a matter of the MasterMix. The mix works perfectly. We are using the SensiFAST Probe Lo-ROX Kit from Bioline.

    Following your advice, today we repeated the DNA extraction leaving a little bit of the aqueous phase with the organic phase and the intermediate layer. To our surprise and happiness, the results were much better than the previous ones. We were able to detect DNA from bacteria infecting seeds at less than 1 UFC/ml of bacteria (we use 10 ml as starting material, which means approx. 7 cell per extraction tube).

    And, What was the difference? this time we kept the interphase with the organic phase. In previous experiments during the DNA isolation we removed the interphase or part of it. If we keep this intermediate phase we get a final pellet very big (DNA and other remains) and not very clean. The samples that we process are from seeds (difficult samples) and we thought that the intermediate layer could contain PCR inhibitors. So, we decided to remove it. This time we decided to retain the intermediate layer with the organic phase to isolate the DNA. Things were much better and no PCR inhibitors were detected. Probably, we were losing a lot of DNA by removing the interphase.

    The today results looks very promising even with difficult samples as seeds.

    I want to thank you the time you have spent trying to solve our problem. Your help has been very valuable for us.

    Sincerely,

    Leandro

    Dr Leandro de Leon, Anove Asociación

    #successstory #Bioline #R&D #Techsupport

    Posted in: Bioline Tags: success storyBiolineR&DTechsupport