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Bioline Launches EPIK miRNA Panel Assays for profiling of cancer samples, biofluids , and stem cells.

By Bioline 9 July 2015

Bioline, The PCR Company, is proud to announce the worldwide release of the EPIK™ miRNA Panel Assays, developed for miRNA profiling of cancer samples, biofluids, and stem cells. The EPIK miRNA Panel Assays are the result of a collaboration between MiRXES in Singapore and Bioline.

Using a novel approach developed by MiRXES, combinations of miRNA specific reverse transcriptase and hemi-nested real-time PCR primers have been designed to maximize miRNA detection sensitivity and specificity. The real-time PCR assays use SensiSMART™ with SYBR® Green to enable detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences. This highly reproducible, sensitive platform has been validated independently by molecular diagnostic and pharmaceutical laboratories and showed superior performance to existing commercial assays. The EPIK miRNA Panel Assays provide researchers with a convenient way to quickly analyze miRNAs that suggest a correlation with the dysregulations in various cancers, in maintenance, differentiation and reprogramming of stem cells and to look at circulating miRNAs that are regarded as a promising new class of biomarkers.

Marco Calzavara, President of Bioline commented,

"The release of the EPIK miRNA Panel Assays follows the release of the ISOLATE II miRNA, Plant miRNA, Biofluids RNA, RNA/DNA/Protein and FFPE RNA/DNA Kits earlier this year and shows the continued commitment of Bioline to produce high quality miRNA kits to help researchers with miRNA expression profiling and quantification and in biomarker discovery."

EPIK™ miRNA Panel Assays confer high sensitivity, specificity and speed to the quantification of miRNAs and have been validated using both synthetic miRNA templates and human total RNA. Typically the assays detect as few as 100 copies of template per RT reaction with excellent assay efficiency and linearity to produce complete systems for miRNA profiling of cancer samples, biofluids and stem cells.

  • Increased sensitivity for limiting amounts of input sample
  • Improved specificity gives superior discrimination of even very closely related targets
  • Faster protocol for earlier results and increased throughput
  • Accurate quantification of low and high expressed targets in the same sample
  • Conveniently formulated to minimize set-up time

Lihan Zhou, CTO of MiRXES, commented,

“This exciting partnership combines our unique miRNA technology and Bioline’s vast experience in qPCR research tools to develop sensitive and robust assays to support the ever-growing community of miRNA researchers”.

MiRXES is a spin-off from the Bioprocessing Technology Institute of the Agency of Science Technology And Research (A*STAR) and the National University of Singapore. MiRXES collaborates extensively with academic, clinical and industrial partners in advancing the science and application of miRNA.

Watch an introduction to EPIK™ Panels.

Find out more about Bioline EPIK™ miRNA Panel Assays

Posted in: BiolinePCRReal-Time PCR Tags: qPCRReal-Time PCRmiRNAEpik

iGEM 2015

By Bioline 12 June 2015

It’s that time of year again!!!! We are proud to announce Bioline is supporting iGEM teams in 2015. Our already competitive pricing drops 40% for the young and enthusiastic iGEM researchers in the United Kingdom.

‘Sponsorship is very difficult, particularly for small projects such as ours. Bioline are very kindly sponsoring us. A big thank you to them.- York iGEM 2014 Team

Popular tools used by successful iGEM teams around the world include:

Get in touch with your local UK Bioline Account Manager to enquire about an iGEM offer pricing plan.

Then, why not let your fellow iGEM researchers know about this great offer using the sharing links below? Also feel free to Tweet or email us if you have any further questions www.bioline.com/social We look forward to hearing from you soon and we wish all iGEM2015 entrants every success in this year's competition!

Terms and Conditions
This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on September 30, 2015. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia.

One-step real-time RT-PCR versus two-step real-time RT-PCR

By Bioline: The PCR Company 20 February 2015 No comments

One-step Vs. two-step real-time RT PCR

Real-Time PCR has become an increasingly popular technique for analysis of gene expression. There are two primary methods of real-time PCR that can be performed. The first involves including the reverse transcriptase step in the same tube as the PCR reaction (one-step).

The second method involves creating cDNA first by means of a separate reverse transcription reaction and then adding the cDNA to the PCR reaction (two-step). There are advantages and disadvantages to both systems that you should considered before choosing the best one for your application, these include the ease of use and cost of reaction to the resulting yield and sequence representation.

Read One-step Vs. two-step real-time RT PCR →

2nd qPCR and Digital PCR Congress 2014, London

By Bioline: The PCR Company 13 September 2014 No comments

Bioline are pleased to be sponsoring and exhibiting at the 2nd qPCR and Digital PCR Congress in London, England on 20th and 21st October.

The meeting brings together key opinion leaders from industry and academia, and covers a range of important topics in real-time PCR, including:

  • MIQE guidelines and standardization
  • Quality control of real-time PCR assays
  • Real-time PCR assay design, optimisation and validation
  • Pathogen detection and quantification
  • Sample preparation and quality control
  • Single cell expression analysis
  • Clinical and drug development applications
  • miRNA, ncRNA, siRNA applications
  • Bioinformatics and data analysis

Case studies in infectious diseases, vaccines, cancer, prenatal diagnosis, diagnostic and clinical applications, microbiology, food microbiology, plant and ecological genomics, as well as other novel applications will be presented.

A special session at the congress will be devoted to plant breeding, plant genomics and applied food and plant pathogen testing:

  • Plant and ecological genomics
  • Detection and identification of plant pathogens
  • Gene expression analysis
  • Real-time PCR in food research
  • Food safety
  • Genetically modified organism (GMO) quantification in food

Bioline maintains a significant real-time PCR research and development facility based in London, the heart of the MedCity biocluster for life science, which includes the new Francis Crick Institute. Bioline maintains a significant real-time PCR research and development facility based in London, the heart of the MedCity biocluster for life science, which includes the new Francis Crick Institute.

See our Your Partner for Plant Research microsite to learn about some of our best tools to enhance your plant research experiments, and download our Plant Research Solutions Guide.

We will be showcasing our latest real-time PCR technological developments at the congress, including the new SensiFAST cDNA Synthesis Kit, a fast, easy-to-use method of generating the highest quality cDNA for highly accurate real-time PCR results.

Bioline will also be highlighting our industrial capabilities for custom manufacturing, including enzymes, buffer chemistries, dyes and internal controls for a wide range of applications from plant genotyping, agribiotech, pathogen testing and cancer research, to biomarkers and diagnostics research.

Our friendly team of real-time PCR specialists will be on-hand at our exhibition booth throughout the event and glad to answer your queries and discuss your research requirements.

For further details of speakers and topics, please see the Global Engage qPCR & Digital PCR Congress Agenda .

We look forward to seeing you at the 2014 congress.

Bioline's Gem of an iGEM Offer is Back for iGEM2014!

By Bioline: The PCR Company 20 June 2014 No comments

Bioline is proud once again to offer its support to teams participating in the 2014 round of the annual iGEM competition. Teams supported by Bioline in previous years have won gold medals and advanced to the finals of the iGEM World Championships.

For a limited time only, all iGEM teams in the United Kingdom can take advantage of up to 40% off already highly-competitive Bioline list prices across our entire product range.

The Must-Have Bioline Toolbox for iGEM Researchers

Popular tools used by successful iGEM teams around the world include:

We used bioline cells but didn’t follow the protocols because the cells were more competent so didn’t need to go through heat shock.Boston iGEM 2012 Team

We also supply a handy range of antibiotics, SOC medium and a rather nifty quick stick ligase for both cohesive and blunt end cloning.

All Bioline products are backed by our friendly and helpful UK based technical support staff, should you need it!

To request full details of our Gem of an iGEM Offer pricing plan, please contact your local Bioline UK account manager using our Rep Finder tool. Then, why not let your fellow iGem researchers know about this great offer using the sharing links below. Also feel free to Tweet or email us if you have any further questions

We look forward to hearing from you soon and we wish all iGEM2014 entrants every success in this year's competition!

PS: You can also read about Bioline’s Synthetic Biology industry partnership with Liverpool Life Sciences UTC, the first school in the United Kingdom specialising in Science and Health Care for 14 to 19 year olds.

Terms and Conditions

This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on October 31, 2014. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia. Bioline products are also available through our carefully selected distributor partners.

Bioline at BioTechnica 2013 – Delivering the latest in direct PCR amplification innovations

By Bioline: The PCR Company 26 September 2013 No comments

Bioline: The PCR Company, a global leader in molecular biology innovation, will be exhibiting (Hall, 9, Stand D26) and presenting (Hall 9, Stand F69) at Europe's leading life science, biotechnology and laboratory show, BioTechnica 2013 (8-10 October, Hannover, Germany). Our Senior Global Product Manager, Dr. Steve Hawkins, will discuss the evolution of direct PCR amplification technologies as part of the prestigious Biotechnica Innovation Forum.

Bioline at Biotechnica 2013The recent increased focus on innovation in biotechnology and medical diagnostics within the world of life sciences has led to important advances in molecular detection and biomedicine. Bioline is proud to be at the forefront of some of the key developments in PCR technologies that are actively accelerating the pace and success of research for scientists around the world.

Analysis of DNA from blood: Technical challenges

Molecular screening of blood samples for blood-borne pathogens, blood cell irregularities and blood group genotyping is a routinely performed process, but can be fraught with stumbling blocks. Some of the key concerns when using PCR to analyse DNA isolated from blood cells include the potential for false-negatives, or poor results that arise from a lack of sensitivity induced by the presence of PCR inhibitors in blood. Common reaction inhibitors include hemoglobin, IgG, lactoferrin, proteases, anticoagulants and salts.

Fast PCR, direct from whole blood, without compromise

Our presentation at the Biotechnica 2013 Innovation Forum is titled 'Fast PCR, Direct From Whole Blood, Without Compromise'. The talk will provide an overview of how technical challenges from direct PCR amplification of blood samples can be overcome using the very latest in direct PCR developments. We will outline an extremely rapid workflow that is specially engineered to overcome various PCR inhibitors typically present in whole blood samples that remove the need for pre-treatment or DNA isolation.

Ultra-fast PCR protocols specially developed to deliver significantly increased sensitivity and PCR success rates, without compromising quality and yield, will also be covered. We will also show how the speed and specificity of these techniques discussed make them the perfect solutions for performing high-throughput end-point multiplex PCR direct from whole blood.

Further details of our upcoming presentation at the Innovation Forum can be found on the Biotechnica 2013 web site.

Visit the brand new Bioline booth at Biotechnica 2013

Throughout the three days of Biotechnica 2013, we'll be presenting all the latest innovations across our endpoint-PCR and real-time PCR product lines. If you're attending, you'll find the brand new Bioline stand in Hall 9, Stand D26 where you will also be able to enter our iPad prize draw. We invite you to experience the power of the latest in life science innovation with Bioline and look forward to seeing you there.

Hands-on Real-Time qPCR Workshop at Birkbeck, sponsored by Bioline

By Bioline: The PCR Company 23 July 2013 No comments

Quantifying nucleic acids by Real-Time qPCR

Hosted by Hosted by qStandard

4th-5th September 2013, Birkbeck, University of London

Register now for a two-day, hands-on workshop sponsored by Bioline: The PCR Company that teaches you the core principles of qPCR and focuses on the practical steps required to design, set-up, validate and analyse Real-Time qPCR assays.

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