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Daisy and Magda, both PhD researchers from Lens Research Lab at the University of Sydney are introduced to PCR and the SensiFAST™ product range.
Review: "We used the ISOLATE II RNA Micro Kit, SensiFAST cDNA Synthesis Kit and SensiFAST SYBR® No-ROX Kit to perform our PCR and got excellent results. Our standard curves were great with r2 values over 0.99. Very impressed and pleased with this result, especially given it was our first time doing real-time PCR. Hope you like the video review we put together!"
To learn more about our SensiFAST range visit www.bioline.com/sensifast
Thank you Tiffany Kosch from James Cook University for your review of SensiFAST™ Probe No-ROX Kit
"I performed a comparison of of Bioline's SensiFAST™ Probe No-ROX Kit (cat. BIO-86005) against several other qPCR master mixes. The Bioline product performed the best overall (i.e. better efficiency and sensitivity, lower cost). I have attached a graph of the Quantitation analysis from my trial run of this product. I have also been very impressed with Bioline customer service and really appreciate that they are willing to send free samples of most of their products."
It’s that time of year again!!!! We are proud to announce Bioline is supporting iGEM teams in 2015. Our already competitive pricing drops 40% for the young and enthusiastic iGEM researchers in the United Kingdom.
‘Sponsorship is very difficult, particularly for small projects such as ours. Bioline are very kindly sponsoring us. A big thank you to them.’ - York iGEM 2014 Team
Popular tools used by successful iGEM teams around the world include:
- Bioline Competent Cells and Cloning Reagents - Transform plasmids from the iGEM kit plates with extremely efficient alpha-select silver or gold efficiency cells.
- MyTaq™ HS - Use MyTaq HS to amplify DNA from your minipreps in as little as 30 minutes, or amplify directly from your colonies! See the MyTaq colony PCR application note or this SOP written by the Danish iGEM2013 team.
- ISOLATE II Plasmid Mini Kit - Pure and very high DNA yields from your transformed colonies.
- VELOCITY DNA Polymerase - Perfect for all your high-fidelity applications.
- HyperLadder™ DNA Markers - Very popular Bioline range of markers for DNA sizing, as used by Craig Venter's research team.
- Real-time PCR Kits - Trusted SensiFAST™ kits for gene expression analysis.
- Core Reagents - High quality buffers, agarose and other essentials.
Get in touch with your local UK Bioline Account Manager to enquire about an iGEM offer pricing plan.
Then, why not let your fellow iGEM researchers know about this great offer using the sharing links below? Also feel free to Tweet or email us if you have any further questions www.bioline.com/social We look forward to hearing from you soon and we wish all iGEM2015 entrants every success in this year's competition!
Terms and Conditions
This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on September 30, 2015. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia.
SensiFAST™ SYBR® Kits have been developed using the latest advances in buffer chemistry and enhancers to ensure unparalleled sensitivity and reaction speed.
- Rapid: unique buffer chemistry for highest specificity and sensitivity
- Sensitive: from low copy targets
- Accurate quantification: hot-start capability reduces primer-dimer formation and saves time
- Flexible: compatible with all standard and fast cycling instruments
Upload and submit your results and we will send you a free Sprint with SensiFAST™ t-shirt!
* Upload your data by June 30, 2015 at bioline.com/sensifastsprint to receive your free SensiFAST™ t-shirt.
Real-Time PCR has become an increasingly popular technique for analysis of gene expression. There are two primary methods of real-time PCR that can be performed. The first involves including the reverse transcriptase step in the same tube as the PCR reaction (one-step).
The second method involves creating cDNA first by means of a separate reverse transcription reaction and then adding the cDNA to the PCR reaction (two-step). There are advantages and disadvantages to both systems that you should considered before choosing the best one for your application, these include the ease of use and cost of reaction to the resulting yield and sequence representation.
Bioline is proud once again to offer its support to teams participating in the 2014 round of the annual iGEM competition. Teams supported by Bioline in previous years have won gold medals and advanced to the finals of the iGEM World Championships.
For a limited time only, all iGEM teams in the United Kingdom can take advantage of up to 40% off already highly-competitive Bioline list prices across our entire product range.
The Must-Have Bioline Toolbox for iGEM Researchers
Popular tools used by successful iGEM teams around the world include:
- Bioline Competent Cells and Cloning Reagents — Transform plasmids from the iGEM kit plates with Bioline’s extremely efficient alpha-select silver or gold efficiency cells.
We used bioline cells but didn’t follow the protocols because the cells were more competent so didn’t need to go through heat shock.— Boston iGEM 2012 Team
- MyTaq™ HS — Use MyTaq HS to amplify DNA from your minipreps in as little as 30 minutes, or amplify directly from your colonies! See the MyTaq colony PCR application note or this SOP written by the Danish iGEM2013 team.
- ISOLATE II Plasmid Mini Kit — Pure and very high DNA yields from your transformed colonies.
- VELOCITY DNA Polymerase — Perfect for all your high-fidelity applications.
- HyperLadder™ DNA Markers — Our hugely popular range of markers for DNA sizing, as used by Craig Venter's research team!
- Real-time PCR Kits — Tried and trusted SensiFAST kits for gene expression analysis.
- Core Reagents — High quality buffers, agarose and other essentials.
All Bioline products are backed by our friendly and helpful UK based technical support staff, should you need it!
To request full details of our Gem of an iGEM Offer pricing plan, please contact your local Bioline UK account manager using our Rep Finder tool. Then, why not let your fellow iGem researchers know about this great offer using the sharing links below. Also feel free to Tweet or email us if you have any further questions
We look forward to hearing from you soon and we wish all iGEM2014 entrants every success in this year's competition!
PS: You can also read about Bioline’s Synthetic Biology industry partnership with Liverpool Life Sciences UTC, the first school in the United Kingdom specialising in Science and Health Care for 14 to 19 year olds.
This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on October 31, 2014. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia. Bioline products are also available through our carefully selected distributor partners.
An alternative way for RNAi libraries to modify the mammalian genome on a large-scale may lie in the use of genome editing technologies such as the use of targeted endonucleases. Such a technique includes Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly-interspaced, short palindromic repeats/CRISPR-associated endonuclease cas9 (CRISPR/Cas9). CRISPR is a Cas9 endonuclease–based method for sequence-specific genome modification. It can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 20-nt targeting sequence within its guide RNA.
These technologies have proven highly useful for gene editing genomes of various species and the speed at which CRISPR techniques have been exploited has been incredibly rapid.
In January 2013 researchers at MIT, the Broad Institute, and Rockerfeller University first reported the new technique based on the CRISPR/Cas9 system, for precisely altering the genomes of living cells through gene addition or deletion. Researchers say the technology could offer an easy-to-use and more cost-effective way to engineer organisms that produce biofuels; to design animal models to study human disease; or to develop new therapies, among other potential applications.
Bioline reagents have been used to augment the workflow in these exciting gene editing technologies to elucidate CRISPR systems, including our SensiFAST™ range of Real-Time PCR Kits, as well as our performance PCR polymerases.
In the first examples of genes that mediate the inhibition of a CRISPR/Cas system, researchers from the University of Toronto identified five distinct ‘anti-CRISPR’ genes in the genomes of bacteriophages infecting Pseudomonas aeruginosa.
Bondy-Denomy, J et al., (2012). Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature 493, 429-432.
Researchers from Minnesota University reported precise, high-frequency editing of 15 genes in pig, goat, and cattle genomes using CRISPR/Cas and TALENs technology. RFLP analysis of colonies were analysed by PCR using MyTaq Red Mix.
Wenfang, T.W. et al., (2013). Efficient nonmeiotic allele introgression in livestock using custom endonucleases. PNAS 110(41), 16526-16531.
To learn more about performance PCR reagents from Bioline, just visit www.bioline.com