CH3-Blue 10^8 Chemically Competent Cells

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CH3-Blue Chemically Competent Cells are provided at a transformation efficiency of 1x108 cfu/µg supercoiled DNA, making them ideal for difficult clone construction or blunt end ligation.

Features & Benefits

  • Versatile -cloning of methylated and non-methylated DNA
  • High efficiency -available in ≥108 cfu/μg, perfect for difficult clone construction
  • Easy screening -with blue/white color selection
  • Convenient size -small aliquotes reduce waste

Applications

  • Cloning of methylated DNA
  • Ideal for subcloning
  • Generating cDNA libraries
  • Blue/white color screening

Description

CH3-Blue Chemically Competent Cells are a highly efficient derivative of E. coli K12, ideal for difficult clone construction using plasmid derived vectors.

To facilitate the cloning of DNA that contains methylcytosine or 5-hydroxymethylcytosine, CH3-Blue lacks the E.coli restriction systems mcrA, mcrBC, mrr and hsdRMS.

The addition of endA1 allows for cleaner preparations of DNA and better results in downstream applications. The elimination of non-specific digestion by Endonuclease I and the recA1 reduces the occurrence of non-specific recombination in cloned DNA. This minimizes recombination events and improves the quality and yield of plasmid DNA.

The lacZ mutation allows blue/white color screening and α-complementation of recombinants.


Notes

Genotype: F- ΔmcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 ara Δ139 Δ(ara, leu)7697 galU galrpsL(StrR) nupG λ-


Components

Reagent

BIO-85039

Chemically Competent Cells

10 x 100µl

pUC19 control vector (10pg/µl)

10µl


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

CH3-Blue 1x108 Chemically Competent Cells are shipped on dry ice







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