Crystal 10x TBE Buffer - Discontinued


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Crystal 10x TBE Buffer is a Tris-Borate-EDTA electrophoresis running buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches.

Features & Benefits

  • Convenient - exactly pre-weighed powder in sealed pouches
  • Easy-to-use - dissolve and use in minutes
  • RNase and DNase free - ideal to standardize DNA electrophoresis
  • Reagents of the highest purity and quality - provides consistent results

Applications

  • Agarose gel electrophoresis of short DNA fragments (<1500bp)

Description

Crystal 10x TBE Buffer is a Tris-Borate-EDTA electrophoresis running buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches. Each pouch contains a pre-determined amount of components, providing increased buffer precision. There is no need to spend time in the laboratory weighing and handling the loose components for the buffers. Simply dissolve the contents of the pouch in deionized water to the recommended volume and the buffer is ready for use without any further preparation.

Bioline Crystal buffers undergo extensive and stringent QC assays and the guaranteed batch-to-batch reproducibility provides consistent results.

One pouch of Crystal 10x TBE buffer dissolved in deionized water and made up to 1000ml yields a 10x stock solution containing 0.89M Tris-Borate, 0.02M EDTA, pH 8.3 at 25°C.

Crystal 10x TBE Buffer is part of our Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.


Components

Reagent

Pouches

10x TBE Buffer

10 x 1000ml


Storage & Stability

Unopened pouches can be stored at room temperature for 3 years. The 10x stock solution prepared in a sterile bottle with high-quality water and filtered through a 0.20μm filter can be stored for 1 month at 4°C. Passing the 10x solution through the filter prevents or delays the formation of precipitates. 1x solutions should be prepared fresh before use.






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