Crystal 1x TG Buffer - Discontinued


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Crystal 1x TG Buffer is a Tris-glycine protein electrophoresis buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches.

Features & Benefits

  • Convenient - exactly pre-weighed powder in sealed pouches
  • Easy-to-use - ideal to standardize protein electrophoresis
  • Pure - formulated from analytical grade reagents
  • Guaranteed batch-to-batch reproducibility - provides consistent results

Applications

  • Protein polyacrylamide gel electrophoresis (PAGE)
  • Western blotting assays

Description


Crystal 1x TG Buffer is a Tris-glycine protein electrophoresis buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches. Each pouch contains a pre-determined amount of components, providing increased buffer precision. There is no need to spend time in the laboratory weighing and handling the loose components for the electrophoresis buffer. Simply dissolve the contents of the pouch in deionized water to the recommended volume and the buffer is ready for use without any further preparation.

Bioline Crystal buffers undergo extensive and stringent QC assays and the guaranteed batch-to-batch reproducibility provides consistent results.

One pouch of Crystal 1x TG buffer dissolved in deionized water and made up to 1000ml yields a solution containing 0.1M Tris and 0.192M glycine, pH 8.3 at 25°C.

Crystal 1x TG Buffer can be used to perform SDS-PAGE when used in combination with the anionic detergent sodium dodecyl sulfate (SDS) at a concentration of 0.1% This can be obtained by dissolving 2 tablets of the Bioline Crystal SDS Reagent in 1000ml of the Trisglycine buffer. Crystal SDS reagent is provided in preweighed 0.5g tablets (BIO-37109).

Crystal 1x TG Buffer is part of our Essentials range of products, which includes agarose, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.


Components

Reagent

Pouches

1x TG Buffer (Powder)

10


Storage & Stability

Unopened pouches can be stored at room temperature for unlimited time. The 1x stock solution prepared in a sterile bottle with high-quality water and filtered through a 0.20μm filter can be stored for 1 month at 4°C. Passing the 1x solution through the filter prevents or delays the formation of precipitates.


Shipping conditions


Shipped at room temperature.






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