Crystal 50x TAE Buffer - Discontinued


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Crystal 50x TAE buffer is a Tris-Acetate-EDTA electrophoresis running buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches.

Features & Benefits

  • Convenient - exactly pre-weighed powder in sealed pouches
  • Easy-to-use - dissolve and use in minutes
  • RNase and DNase free - ideal to standardize DNA electrophoresis
  • Reagents of the highest purity and quality - provides consistent results

Applications

  • Agarose gel electrophoresis of long nucleic acid fragments (>1500bp)
  • Native and denaturing RNA analysis
  • Southern blotting

Description

Crystal 50x TAE buffer is a Tris-Acetate-EDTA electrophoresis running buffer made from reagents with the highest purity and delivered in convenient ready-to-use sealed pouches.

Each pouch contains a pre-determined amount of components, providing increased buffer precision. There is no need to spend time weighing and handling loose components for buffers. Simply dissolve the contents of each pouch in deionized water, to the recommended volume, and the buffer is ready for use without any further preparation.

Crystal 50x TAE Buffer is recommended for large fragments (above 1500bp) and complex mixtures of large genomic DNA. TAE has a lower buffering capacity than TBE, however linear dsDNA tends to migrate faster in TAE than in TBE.

Crystal 50x TAE Buffer is also used in non-denaturing RNA agarose gel electrophoresis. Southern blots are generally derived from gels prepared and run in TAE buffers. TAE buffers are considered better for gel purification since acetate is less inhibitory then borate for downstream applications.

Bioline Crystal buffers undergo extensive and stringent QC assays and the guaranteed batch-to-batch reproducibility provides consistent results.

One pouch of Crystal 50x TAE buffer dissolved in deionized water and made up to 1000ml yields a 10x stock solution containing 2.0M Tris-Acetate, 0.05M EDTA, pH 8.3 at 25°C.

Crystal 50x TAE Buffer is part of our Essentials range of products, which includes agarose, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.


Components

Reagent

Pouches

50x TAE Buffer

5 x 1000ml


Storage & Stability

Unopened pouches can be stored at room temperature for 3 years.

The 50x stock solution prepared in a sterile bottle with high-quality water and filtered through a 0.20μm filter can be stored for 1 year at room temperature.


Shipping conditions

Shipped at ambient temperature.







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