EasyLadder I

Cat No. Size List Price* Qty  
BIO-33045 200 Lanes
BIO-33046 500 Lanes
*For more information on pricing please contact us

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EasyLadder I is a ready-to-use DNA molecular weight marker, ideal for short runs (1cm to 3cm) on agarose gels. The ladder is pre-mixed with red loading buffer.

Features & Benefits

  • Easy size determination
  • 5 bands from 100bp - 2000bp
  • Ready-to-use format
  • Optional mass determination


  • Ideal for short runs
  • Ideal for high-throughput applications


EasyLadder I is a ready-to-use DNA molecular weight marker, especially designed for DNA analysis in standard and high-throughput agarose gels. The ladder is pre-mixed with red loading buffer.

EasyLadder I contains 5 even-intensity bands ranging from 100 to 2000bp for easy identification of the DNA samples analyzed. EasyLadder I is ideal for short runs (1 to 3cm) on 1% to 2% agarose gels.

For the best results we recommend using Bioline Agarose or Bioline Agarose Tablets.



100 Lanes

200 Lanes

500 Lanes

EasyLadder I


2 x 500µl

5 x 500µl

5x Sample Loading Buffer




Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

When stored at +4°C, EasyLadder I can be stored for up to 12 months. When stored at room temperature, EasyLadder I can be stored for up to 1 month

Shipping conditions

Ambient temperature.

Certificates of Analysis (COAs)

Certificates of Analysis for EasyLadder I are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 25 COAs for EasyLadder I →

Frequently asked questions

  • What are the optimal conditions for running my EasyLadder?

      Please see individual product insert (available online and included with each product) for the concentration of Agarose each ladder should be run on. The smaller the DNA product, the higher the percentage of Agarose required in the gel. All HyperLadders and EasyLadders are ready-to-load, and require no addition of loading buffer prior to use.
  • My EasyLadder does not look as it should when I run it, what should I do?

      Problems with our EasyLadder are extremely rare, so please make sure you have carried out each of the following correctly:
      • Used the correct percentage of Agarose in your gel for the specific marker in question.
      • Used 5µl of the marker per lane.
      • Used the correct concentration of stain (e.g. EtBr) to visualise correctly.
      • Vortexed the marker gently before use.
      Should problems persist, please contact technical services for further assistance.
  • Do I need to pre-treat my EasyLadder prior to loading?

      No pre-treatment, such as denaturing, is necessary prior to use.
  • Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?

      Our HyperLadder and EasyLadder range is designed for use on Agarose gels, and we do not recommended them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder 25bp, our small fragment DNA marker, would be the most appropriate.
  • We do not wish to stain our gels using EtBr, will other stains still resolve the EasyLadder correctly?

      Other stains, such as SYBR® Green Gel Stain, can be used with our ladders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the EasyLadder remains quantitative.
  • Do I need to add loading buffer to my Bioline marker to load it onto my gel?

      No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.

Other researchers use:

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