HyperLadder™ 500bp - Discontinued


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HyperLadder™ 500bp is a ready-to-use molecular weight marker, specially designed for easy size determination. The ready to- use format reduces handling steps and saves time; simply transfer the HyperLadder from the vial to the gel.

Features & Benefits

  • Easy size determination - 11 bands from 500bp - 5000bp
  • Contains red dye - for direct gel loading
  • Several higher intensity bands - for easy orientation
  • Easy storage - stable for 6 months at room temperature

Description

HyperLadder™ 500bp is a ready-to-use molecular weight marker, specially designed for easy size determination. This ready to- use format reduces handling steps and saves time; simply transfer the HyperLadder from the vial to the gel.

HyperLadder 500bp produces a pattern of 11 regularly spaced bands, ranging from 500 to 5000bp. To allow easy identification and orientation, the 1000 and 3500bp bands have the highest intensity. HyperLadder 500bp contains a red dye, migrating at 900bp in 1.5% agarose gel.

Optionally the HyperLadder can be used for mass determination. A 5x sample loading buffer is supplied for your convenience. See our HyperLadder Selection Guide page for product comparisons of the complete HyperLadder range.

For the best results when using our HyperLadder range we recommend using Bioline Agarose or Agarose Tablets.


Components

Reagent

100 Lanes

200 Lanes

500 Lanes

HyperLadderTM 500bp

500µl

2 x 500µl

5 x 500µl

5x Sample Loading Buffer

1ml

1ml

1ml


Storage & Stability

All components are shipped at ambient temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Gentle vortexing is recommended prior to use. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended. When stored at +4°C the product is stable for 4 months.


Shipping conditions

At ambient temperature.


Certificates of Analysis (COAs)

Certificates of Analysis for HyperLadder™ 500bp - Discontinued are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 1 COAs for HyperLadder™ 500bp - Discontinued →


Frequently asked questions

  • What are the optimal conditions for running my HyperLadder?

      Please see individual product insert available online and included with each product for the concentration of Agarose each ladder should be run on. The smaller the DNA product, the higher the percentage of Agarose required in the gel. All HyperLadders are ready-to-load, and require no addition of loading buffer prior to use.
  • My HyperLadder doesn't look as it should when I run it, what should I do?

      Problems with our HyperLadders are extremely rare, so please make sure you have carried out each of the following correctly:
      • Used the correct percentage of Agarose in your gel for the specific marker in question.
      • Used 5µl of the marker per lane.
      • Used the correct concentration of stain (e.g. EtBr) to visualise correctly.
      • Vortexed the marker gently before use.
      Should problems persist, please contact technical services for further assistance.
  • Do I need to pre-treat my HyperLadder prior to loading?

      No pre-treatment, such as denaturing, is necessary prior to use.
  • Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?

      Our HyperLadder range is designed for use on Agarose gels, and we do not recommended them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder™ 25bp, our small fragment DNA marker, would be the most appropriate.
  • We do not wish to stain our gels using EtBr, will other stains still resolve the HyperLadder correctly?

      Other stains, such as SYBR® Green Gel Stain, can be used with our HyperLadders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder remains quantitative.
  • Do I need to add loading buffer to my Bioline marker to load it onto my gel?

      No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.
  • 7. How is one unit of activity of a polymerase defined?

      One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.

  • 8. What are the advantages of using a 2x Mix rather than setting up a PCR reaction from scratch?

      All Bioline polymerases are available in the convenient format of a 2x master mix, containing all the reagents and additives necessary to perform successful PCR, with the exception of template, primers and water.

      This formulation not only provides a convenient and hassle-free PCR setup, but also significantly reduces the chances of human error, inaccuracies and contamination by reducing the pipetting steps required.

  • 9. What is the concentration of the polymerase mixes?

      All Bioline PCR mixes come supplied at a 2x concentration, requiring the user simply to add template, primers and PCR grade water to a final concentration of 1x.

  • 10. What is the recommended reaction volume of the polymerase mixes?

      We recommend a final reaction volume of 50µl, this requiring the use of 25µl of the 2x master mix, and to make up to 50µl using template, primers and PCR grade water.

  • 11. What is the composition of the polymerase mixes?

      Whilst the exact composition of the mixes is proprietary information, all mixes are made up of Reaction Buffer, Magnesium, dNTP's, Polymerase and additives, designed to keep the polymerase stable in the mix, as well as provide the optimal conditions for PCR.




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