ImmoMix™ Red

Cat No. Size List Price* Qty  
BIO-25022 500 x 50µl Reactions $780.00
*For more information on pricing please contact us

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ImmoMix™ is a complete ready-to-use, red-colored, high yield; heat-activated, 2x reaction-mix which simply requires the user to add water, template and primers, and then pre-heat to 95°C for 10 minutes to carry out successful PCR assays. Once completed, the reaction mix can be loaded directly on to a gel.

Features & Benefits

  • Convenient - pre-mixed, pre-optimized 2x solution
  • Ready to use format - reduces risk of contamination
  • Fast set up - decreased time compared to traditional methods
  • Red color - indicates the mix has been added
  • Direct gel loading - no further processing necessary


  • Ultra-high specificity for multiplex reactions
  • Products suitable for TA cloning


ImmoMix™ Red is a complete ready-to-use heat-activated 2x reaction-mix, which simply requires the user to add only water, template and primers, and then pre-heat to 95°C for 10 minutes to successfully carry out PCR assays. The 10 minute activation step eliminates the presence of non-specifics such as primer-dimers and mis-primed products, since the enzyme is inactive at initial low temperatures.

ImmoMix Red combines all of the features and advantages of ImmoMix and contains an additional inert red dye. This non-toxic, non-hazardous red dye allows users to load samples directly onto a gel, without the need to add loading buffer since the mix is of sufficiently high density to sink to the bottom of the gel.

Adequate mixing is also ensured when reactions are set up. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).

ImmoMix Red is based on IMMOLASE™ DNA Polymerase, which leaves an ´A´ overhang and can be used for a wide variety of templates. An additional 50mM MgCl2 solution is included should any fine adjustments be required.

ImmoMix Red reduce the time needed to set up reactions, thereby reducing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to pipetting errors.


ImmoMix is a trademark of Bioline.



500 Reactions

ImmoMix Red

10 x 1.25 ml

50mM MgCl2 Solution



  • BIO-25021: 100 x 50µl Reactions 2 x 1.25ml
  • BIO-25022: 500 x 50µl Reactions 10 x 1.25ml


  • 2x

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Components may also be stored at +4°C and will remain stable for up to 2 weeks, however storage at -20°C is recommended.

Shipping conditions

On Dry Ice or Blue Ice.

Certificates of Analysis (COAs)

Certificates of Analysis for ImmoMix™ Red are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 15 COAs for ImmoMix™ Red →

Frequently asked questions

  • Which polymerase do I need for my specific application?

      At Bioline we pride ourselves in supplying high-quality polymerases to suit your requirements. To aid your selection of the most suited enzyme for your specific applications, please see our enzyme selection tool.

  • What are the storage conditions and stabilities of Bioline polymerases?

      All our polymerases are guaranteed for a period of 12 months from the date of purchase. These should be stored at -20°C during this time for optimal retention of activity.
      Please Note: We do not recommend the storage of our polymerases at -80°C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
  • I am having problems optimizing my PCR, what would you recommend?

      PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

      Observation Recommended Solution(s)
      No or low PCR yield  For difficult templates (AT and GC rich).
       Enzyme concentration too low – increase the amount enzyme in 0.5U increments.
       Magnesium concentration too low – increase concentration in 0.25mM increments with a starting  concentration of 1.75mM.
       Primer concentration not optimised. Titrate primer concentration (0.3-1µM); ensuring that both  primers have the same concentration.
       Template concentration too low – Increase Concentration of template
       Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or  contaminated
      Multiple Bands  Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be  at least 5°C below the calculated Tm of primers.
       Prepare master mixes on ice or use a heat-activated polymerase.
       For problems with low specificity. Try Hi-Spec Additive or 2x PolyMate (not supplied) to improve  specificity.
      Smearing or artifacts  Template concentration too high. Prepare serial dilutions of template.
       Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.
       Enzyme concentration too high - decrease the amount of enzyme in 0.5U increments.
       Extension time too long. Reduce extension time in 0.5-1 minute increments.

  • What do the terms yield, fidelity, processivity and specificity actually mean?

      These terms refer to parameters to be considered when performing PCR, and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial:
      Yield: The amount of DNA produced in a PCR reaction
      Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand.
      Processivity: The length of time a polymerase is associated with the template, and therefore the size of fragment which can be amplified.
      Specificity: A measure of the unwanted by-products generated in a reaction.
  • What is a proofreading polymerase?

      In nature, the ability of a DNA polymerase to correct misincorporations of nucleotides in the DNA strand being elongated is often crucial to the survival of the host organism. This ability is termed "proofreading activity", and occurs in the 3' to 5' direction. This activity also leads to the polymerase removing unpaired nucleotides overhanging at the 3'end (A-overhangs), creating blunt ends.
  • What are the error rates of Bioline DNA Polymerases?

      Please see the following table for the appropriate error rates:

      BIOLASE, IMMOLASE, MangoTaq, MyTaq DNA Polymerase
       1.1 x 10-4 base substitutions/bp (Tindall and Kunkel, 1988)
       2.4 x 10-5 frameshift mutations/bp (Tindall and Kunkel, 1988)
       2.1 x 10-4 errors/bp (Keohavang and Thilly, 1989)
       7.2 x 10-5 errors/bp (Ling et al., 1991)
       8.9 x 10-5 errors/bp (Cariello et al., 1991)
       2.0 x 10-5 errors/bp (Lundberg et al., 1991)
       1.1 x 10-4 errors/bp (Barnes, 1992)
      ACCUZYME  1.6 x 10-6 errors/base (Lundberg et al., 1991).

  • How is one unit of activity of a polymerase defined?

      One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.
  • What are the advantages of using a 2x Mix rather than setting up a PCR reaction from scratch?

      All Bioline polymerases are available in the convenient format of a 2x master mix, containing all the reagents and additives necessary to perform successful PCR, with the exception of template, primers and water.
      This formulation not only provides a convenient and hassle-free PCR setup, but also significantly reduces the chances of human error, inaccuracies and contamination by reducing the pipetting steps required.
  • What is the concentration of the polymerase mixes?

      All Bioline PCR mixes come supplied at a 2x concentration, requiring the user simply to add template, primers and PCR grade water to a final concentration of 1x.
  • What is the recommended reaction volume of the polymerase mixes?

      We recommend a final reaction volume of 50µl, this requiring the use of 25µl of the 2x master mix, and to make up to 50µl using template, primers and PCR grade water.
  • What is the composition of the polymerase mixes?

      Whilst the exact composition of the mixes is proprietary information, all mixes are made up of Reaction Buffer, Magnesium, dNTPs, Polymerase and additives, designed to keep the polymerase stable in the mix, as well as provide the optimal conditions for PCR.
  • What is the final working concentration (at 1x) of MgCl2 in each mix?

      Bioline's mixes contain Magnesium Chloride at the following concentrations (please see table below). An additional tube of 50mM MgCl2 solution is supplied with each mix to facilitate further optimization if required.

       Bioline Mix  Magnesium Concentration.
      ACCUZYME Mix  2.0mM.
      BioMix  2.0mM.
      BioMix Red  2.0mM.
      BIO-X-ACT Long Mix  2.0mM.
      MangoMix  3.0mM.
      MyTaq  3.0mM.
      RANGER  1.5mM.

Other researchers use:

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