IPTG

Cat No. Size List Price* Qty  
BIO-37036 5g
$123.00
 
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IPTG (Isopropyl ß-D-1-thiogalactopyranoside), is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

Features & Benefits

  • Induces E.coli lac operon activity - suitable for cloning and protein expression procedures
  • Dioxane free - does not disrupt normal cell function
  • >99.6% purity - confirmed by HPLC
  • Convenient - available as powder and stabilized stock solution

Applications

  • Blue/white color screening
  • Induction of lac operon for protein expression
  • Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG

Description

Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG.

IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.


Components

Reagent

BIO-37036

IPTG Powder

5g


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for IPTG are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 6 COAs for IPTG →





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