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    Highly-efficient purification of genomic DNA from whole blood, cultured cells, serum, plasma or other body fluids by combining the stringency of guanidinium thiocyanate lysis with the speed and purity of silica-membrane column purification.

    Product Highlights

    • Fast – simple extraction of high purity genomic DNA, ideal for use in all applications, in as little as 30 minutes
    • High-performance – recovery of consistently high quality genomic DNA from whole blood, cultured cells, serum, plasma and other body fluids
    • Efficient – optimized lysis conditions and column matrix for improved recovery of genomic DNA from each sample
    • Versatile – recovery of host genomic DNA, bacterial and viral DNA from human and animal blood samples
    • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation

    Product Description

    The ISOLATE II blood DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from blood, without the need for hazardous reagents such as phenol.

    By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Blood DNA Kit provides a fast method for the purification of high-quality genomic DNA from whole blood, cultured cells, serum, plasma and other human / animal body fluids treated with EDTA, citrate or heparin. It is also possible to purify bacterial and viral DNA (e.g. HBV) from blood samples.

    The ISOLATE II Blood DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.


    • qPCR
    • End-point PCR
    • Multiplex PCR
    • Southern, dot and slot blotting
    • Genotyping
    • Next Generation Sequencing
    • Genetic testing
    Fast purification of high-quality genomic DNA from whole blood, cultured cells, serum, plasma or other body fluids.

    Nucleic Acid Isolation Guide

    Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research





    10 Preps

    50 Preps

    250 Preps

    ISOLATE II Blood DNA Spin Columns & Collection Tubes




    Collection Tubes (2 mL)




    Buffer G3

    10 mL

    50 mL

    60 mL

    Wash Buffer GW1

    6 mL

    30 mL

    150 mL

    Wash Buffer GW2 (concentrate)

    6 mL

    12 mL

    50 mL

    Elution Buffer G

    13 mL

    13 mL

    60 mL

    Proteinase K (lyophilized)

    6 mg

    30 mg

    2 x 75 mg

    Proteinase K Buffer PR

    1.8 mL

    1.8 mL

    8 mL

    Bench Protocol Sheet




    Storage & Stability

    All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    Ambient temperature.


    The columns supplied with the ISOLATE II kits may appear similar, but each type has been optimized to work within the buffer system supplied with the corresponding kit. The swapping of columns (or buffers) between kits may lead to no recovery of nucleic acid whatsoever, or at the very least severely impaired purification.

    Interruption of the DNA/RNA extraction process is possible after the sample lysis only. It is possible to homogenize and lyse the samples and to store them in the freezer until use for RNA extraction. RNA clean up with a column cannot be interrupted and we recommend to avoid delays during the column purification process. If a delay is unavoidable the columns should be stored on ice.

    The elution buffer is ideal for applications such as restriction enzyme digestion, sequencing and PCR. It is possible to use DNase-free water for elution, but you should expect a slightly lower yield.

    The elution buffer of this kit does not contain EDTA which could interfere with downstream applications like PCR or sequencing.

    It is not recommended to exceed the maximum volume to prevent clogging of the column. It is possible to split the sample and work with several columns in parallel.