ISOLATE Plant RNA Mini Kit - Discontinued


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This product has been discontinued.
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ISOLATE Plant RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of plant tissues, including leaves, bark, roots, fruits, etc. Up to 100mg starting material can be processed per spin column.

Features & Benefits

  • Rapid protocol - 30 minutes after homogenization
  • High purity RNA - ready for downstream applications
  • Extra filters (shredders) included - to enhance sample homogenization
  • Two optimized extraction buffers - for all plant samples

Description

ISOLATE Plant RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of plant tissues, including leaves, bark, roots, fruits, etc. Up to 100mg starting material can be processed per spin column.

The protocol is easy to follow on a step-by-step basis. Two lysis buffers are provided to ensure lysis of different sample types. The buffers also inactivate RNases, thus protecting the released RNA. The lysate is applied to a spin column to selectively remove genomic DNA, eliminating the need to perform a separate DNase digestion step. The RNA is then bound to a silica membrane. Subsequent wash steps remove the remaining cell debris and pure RNA is eluted in the final step with RNase-free water.

The isolated RNA shows excellent performance in downstream applications such as RT- PCR (fig. 1), reverse transcription (fig. 2), Northern blot analysis, microarrays and RNA protection assays.

Components

Reagent

10 Reactions

50 Reactions

250 Reactions

ISOLATE II Filters

10

50

 250

ISOLATE II RNA Mini Columns

10

50

 250

Collection Tubes (2ml)

30

150

750 

Collection Tubes (1.5ml)

10

50

250 

Lysis Buffer RLY

10ml

25ml

125ml

Wash Buffer RW1

15ml

15ml

80ml

Wash Buffer RW2

5ml

12.5ml

3 x 25ml

Membrane Desalting Buffer MEM

10ml 

25ml 

125ml 

Reaction Buffer for DNase I RDN

3ml 

7ml 

30ml 

DNase I, RNase-free (lyophilized)

1 vial

1 vial 

5 vials

 RNase-free water

5ml

15ml

65ml 

Product Manual

 1

 1

 1

Bench Protocol Sheet

 1

 1

 1


Storage & Stability

ISOLATE Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable for 12 months.

Shipping conditions


Room temperature.







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