ISOLATE Plasmid Mini Kit - Discontinued


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This product has been discontinued.
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ISOLATE Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using proprietary filter membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria with clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants such as cellular debris, RNA and salts are efficiently removed during the washing steps. In the final step, highly pure plasmid DNA is eluted in a low-salt buffer.

Features & Benefits

  • Rapid protocol: 15 minutes/5 preps
  • High purity plasmid DNA: typical A260/A280 ratio >1.8
  • High yields: up to 13μg from 5ml culture
  • Isolated DNA is ready for downstream applications

Description

ISOLATE Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using proprietary filter membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria with clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants such as cellular debris, RNA and salts are efficiently removed during the washing steps. In the final step, highly pure plasmid DNA is eluted in a low-salt buffer. The kit shows excellent recovery of plasmid DNA even from low culture volumes (fig. 1) and reproducible results (fig. 2).

Separate protocols are provided for the isolation of high copy and low copy plasmids. In each case, intact, high purity plasmid DNA is isolated within 15 minutes. Typically, up to 13μg and 18μg plasmid DNA is obtained from 5ml and 10ml cultures, respectively.

The isolated DNA shows excellent performance in downstream molecular biology applications such as PCR, transformation, cloning, sequencing (fig. 3), restriction analysis, etc.

Components

Reagent

10 Reactions

50 Reactions

250 Reactions

ISOLATE II Plasmid Mini Spin Columns

10

50

 250

Collection Tubes

10

50

 250

Resuspension Buffer P1

5ml

15ml

75ml

Lysis Buffer P2

5ml

15ml

3 x 25ml 

Neutralization Buffer P3

5ml

20ml

100ml

Wash Buffer PW1

6ml

30ml

2 x 75ml

Wash Buffer PW2

2ml

2 x 6ml

2 x 20ml

Elution Buffer P

10ml 

25ml 

75ml 

RNase A (lyophilized)

2mg

6mg 

30mg

Product Manual

 1

 1

 1

Bench Protocol Sheet

 1

 1

 1


Storage & Stability

ISOLATE Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable until the expiry date on the outer box label.

Shipping conditions


Room temperature.







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