ISOLATE RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of samples. The kit is compatible with animal tissues, cultured cells and bacterial cells.
The cells are lysed with an optimized lysis buffer, which simultaneously inactivates RNases thus protecting the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA. There is no need to perform a separate DNase digestion step. The RNA is then bound to a silica membrane. Subsequent wash steps remove the remaining cell debris and pure, high quality RNA is eluted in the final step with RNase-free water (Fig. 1).
The isolated RNA shows excellent performance in downstream applications such as reverse transcription, real-time PCR (Fig. 2), Northern blot analysis, microarrays and RNA protection assays.