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    Cat. No.
    Size
    List Price*
    QTY
    BIO-37102
    10ml
    $115.00
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    Description

    The 5x MangoTaq™ Colored Reaction Buffer contains two inert dyes (red and orange) that separate during gel electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel.

    Product Highlights

    • Optimized 5x reaction buffer for PCR
    • Two inert dyes for easy orientation during DNA electrophoresis

    Product Description

    The 5x MangoTaq™ Colored Reaction Buffer contains two inert dyes (red and orange) that separate during gel electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The red dye migrates at 300bp on a 2% agarose gel. The orange dye migrates at 30bp on a 2% agarose gel and <10bp on a 1% gel.

    The colored reaction buffer can be loaded directly onto an agarose gel for analysis, without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist. For high-throughput applications, the colored reaction buffer and MangoTaq DNA Polymerase make an ideal choice, since this combination enables the user to load the PCR products directly on a gel to allow visual recognition.

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    Specification

    Components

    Reagent

    BIO-37102

    5x MangoTaq™ Colored Reaction Buffer

    10ml

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.



    FAQs

    No, the dyes and composition of MangoTaq Reaction Buffer are such that the samples will sink easily into the well and the samples can be clearly seen, thus no loading buffer is required to load your samples on an agarose gel.

    The two colored dyes present in this buffer are completely inert and thus will have no effect on downstream applications. An exception is the quantification of PCR products in colored buffers with photometric methods or fluorescence assays. We cannot exclude the impact of these dyes on quantification results and suggest the purification of these samples. If you are concerned about these dyes interfering in your specific applications, we would recommend the cleanup of your samples using the ISOLATE II PCR & Gel Kit or SureClean Plus.