MangoTaq™ DNA Polymerase

Cat No. Size List Price* Qty  
BIO-21083 1000 Units
BIO-21082 2000 Units
BIO-21078 5000 Units
*For more information on pricing please contact us

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MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.

MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang, resulting in PCR product suitable for effective integration into TA cloning vectors.

Features & Benefits

  • Direct gel loading - no need for further post-PCR processing steps
  • Easy visual recognition- reduces pipetting errors
  • Robust performance - perfect for a wide range of PCR reactions
  • Reproducible results - consistent QC ensures reliability


  • High throughput applications
  • Suited to a wide range of PCR assays
  • Products suitable for TA cloning


MangoTaq™ DNA Polymerase offers high yield across a wide range of DNA concentrations (fig. 1 and 2). MangoTaq DNA Polymerase leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

The polymerase is supplied with two different reaction buffers for greater flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.

The two reaction buffers supplied are: 5x Colored Reaction Buffer and 5x Colorless Reaction Buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although extremely rare exceptions may exist.

Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection.


MangoTaq is a trademark of Bioline.



1000 Reactions

2000 Reactions

5000 Reactions



2 x 200μl

5 x 200μl

5x MangoTaq Colored Reaction Buffer

4 x 1.5ml

8 x 1.5ml

20 x 1.5ml

5x MangoTaq Colorless Reaction Buffer

4 x 1.5ml

8 x 1.5ml

20 x 1.5ml

50mM MgCl2 Solution

2 x 1.2ml

4 x 1.2ml

10 x 1.2ml



Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

On Dry Ice or Blue Ice.

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C

Certificates of Analysis (COAs)

Certificates of Analysis for MangoTaq™ DNA Polymerase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 19 COAs for MangoTaq™ DNA Polymerase →

Frequently asked questions

    1. Which polymerase do I need for my specific application?
    At Bioline we pride ourselves in supplying high-quality polymerases to suit your requirements. To aid your selection of the most suited enzyme for your specific applications, please see our polymerase selection guide.

    2. What are the storage conditions and stabilities of Bioline polymerases?
    All our polymerases are guaranteed for a period of 12 months from the date of purchase. These should be stored at
    -20°C during this time for optimal retention of activity.
    Please Note: We do not recommend the storage of our polymerases at -80°C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.

    3. I am having problems optimizing my PCR, what would you recommend?
    PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

    Observation Recommended Solution(s)
    No or low PCR yield  For difficult templates (AT and GC rich).
     Enzyme concentration too low – increase the amount enzyme in 0.5U increments.
     Magnesium concentration too low – increase concentration in 0.25mM increments with a starting  concentration of 1.75mM.
     Primer concentration not optimised. Titrate primer concentration (0.3-1µM); ensuring that both  primers have the same concentration.
     Template concentration too low – Increase Concentration of template
     Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or  contaminated
    Multiple Bands  Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be  at least 5°C below the calculated Tm of primers.
     Prepare master mixes on ice or use a heat-activated polymerase.
     For problems with low specificity. Try Hi-Spec Additive or 2x PolyMate (not supplied) to improve  specificity.
    Smearing or artifacts  Template concentration too high. Prepare serial dilutions of template.
     Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.
     Enzyme concentration too high - decrease the amount of enzyme in 0.5U increments.
     Extension time too long. Reduce extension time in 0.5-1 minute increments.

    As an alternative for quick and simple PCR optimization, we recommend using SureBand PCR Optimization kit.

    4. What do the terms yield, fidelity, processivity and specificity actually mean?
    These terms refer to parameters to be considered when performing PCR, and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial:

    • Yield: The amount of DNA produced in a PCR reaction
    • Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand.
    • Processivity: The length of time a polymerase is associated with the template, and therefore the size of fragment which can be amplified.
    • Specificity: A measure of the unwanted by-products generated in a reaction.

    5. What is a proofreading polymerase?
    In nature, the ability of a DNA polymerase to correct misincorporations of nucleotides in the DNA strand being elongated is often crucial to the survival of the host organism. This ability is termed “proofreading activity”, and occurs in the 3’ to 5’ direction. This activity also leads to the polymerase removing unpaired nucleotides overhanging at the 3’end (A-overhangs), creating blunt ends.

    6. What are the error rates of Bioline DNA Polymerases?
    Please see the following table for the appropriate error rates:

    BIOTAQ, IMMOLASE, MangoTaq, MyTaq DNA Polymerase  1.1 x 104 base substitutions/bp (Tindall and Kunkel, 1988)
     2.4 x 105 frameshift mutations/bp (Tindall and Kunkel, 1988)
     2.1 x 104 errors/bp (Keohavang and Thilly, 1989)
     7.2 x 105 errors/bp (Ling et al., 1991)
     8.9 x 105 errors/bp (Cariello et al., 1991)
     2.0 x 105 errors/bp (Lundberg et al., 1991)
     1.1 x 104 errors/bp (Barnes, 1992)
    ACCUZYME  1.6 x 106 errors/base (Lundberg et al., 1991).
    VELOCITY  4.4 x 10-7 errors/base (Frey & Suppmann, 1995).

    7. How is one unit of activity of a polymerase defined?
    One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.

    8. What is the concentration of MangoTaq DNA Polymerase?
    MangoTaq is supplied at 1U/µl.

    9. When using MangoTaq DNA Polymerase, is there a need to use loading buffer when loading PCR samples onto a gel?
    No, the dyes and composition of MangoTaq Reaction Buffer are such that the samples will sink easily into the well, and the samples can be clearly seen, thus no loading buffer is required to load your samples on an agarose gel.

    10. Will the dyes present in MangoTaq Reaction Buffer interfere with further post-PCR experiments?
    The two colored dyes present in this buffer are completely inert, and thus will have no effect on downstream applicationsIf you are concerned about these dyes interfering in your specific applications, we would recommend the cleanup of your samples using SureClean Plus.

Customer Testimonials

"The best performance was exhibited by the MangoTaq DNA polymerase, which was the only polymerase which was able to amplify the 620bp amplification product from the 102 year old sample."

Other researchers use:

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