MyFi™ Mix

Cat No. Size List Price* Qty  
BIO-25049 100 x 50µl Reactions
BIO-25050 500 x 50µl Reactions
*For more information on pricing please contact us

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MyFi™ is a novel, antibody-mediated DNA polymerase complex with enhanced sensitivity and fidelity, ideally suited to problematic DNA templates.

Features & Benefits

  • Convenient - ready-to-use, all-in-one 2x mastermix
  • New generation DNA polymerase complex - provides robust PCR with 3.5-fold higher fidelity than wild-type Taq polymerase
  • Antibody-based hot-start polymerase - enhances specificity and allows room-temperature reaction set-up
  • High processivity - efficiently amplifies complex mammalian DNA fragments up to 10kb
  • Novel 5x buffer system - ensures reliability, reproducibility and convenience


  • TA Cloning
  • Long PCR
  • Low-copy PCR assays
  • PCR of difficult DNA templates (problematic GC/AT-rich genomic DNA)
  • High-fidelity PCR assays (library amplification)


MyFi Mix is a ready-to-use 2x mix formulation, containing all the components (including proprietary enhancers and stabilizers) necessary for trouble-free PCR reaction assembly, while delivering the same unique balance of PCR sensitivity and high-fidelity. The pre-optimized MyFi Mix formulation, supplied in a convenient single tube, reduces the number of pipetting steps improving throughput and reproducibility (Fig. 1).

MyFi Mix has been developed with an industry-leading, novel buffer chemistry containing pre-optimized concentrations of ultra-pure dNTPs, MgCl2 and proprietary PCR enhancers for exceptional versatility across a range of challenging assays including long PCR, low-copy PCR and amplification of problematic GC and AT-rich genomic templates. MyFi Mix generates extremely high DNA yields and produces 3'-A overhangs which are ideal for direct integration into TA-cloning vectors. In addition, MyFi Mix provides the convenience of room temperature reaction assembly and avoids non-specific amplification and primer-dimer formation.


MyFi is a Trademark of Bioline.



100 Reactions

500 Reactions

MyFi Mix, 2x

2 x 1.25ml

10 x 1.25ml


  • BIO-25049: 100 x 50µl Reactions: 2 x 1.25ml
  • BIO-25050: 500 x 50µl Reactions: 10 x 1.25ml


  • 2x

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on Dry Ice or Blue Ice.

Certificates of Analysis (COAs)

Certificates of Analysis for MyFi™ Mix are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 27 COAs for MyFi™ Mix →

Customer Testimonials

"MyFi Mix is more sensitive than our current Taq and so easy to use/set up. Nice product."
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"MyFi has higher yield (than HiFi Hot-Start) of the desired amplicons."
"MyFi gave a decent PCR product at this condition while there was no amplification from ExTaq."
"APEX master mix failed to amplify the expected product, yet MyFi successfully amplified a band at the expected size, with reduced non-specific amplicons at higher annealing temperatures."

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