MyTaq™ Extract-PCR Kit

Cat No. Size List Price* Qty  
BIO-21126 100 Reactions
$188.00
 
BIO-21127 500 Reactions
$804.00
 
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MyTaq Extract-PCR Kit provides quick and easy extraction and amplification of DNA from a variety of tissue types. The MyTaq Extract-PCR Kit maximizes sensitivity while simultaneously minimizing contamination risks to deliver greater experiment success rates.

Features & Benefits

  • Rapid extraction protocol - high-yield, PCR-ready DNA in 15 minutes
  • Easy-to-use - eliminates complicated DNA extraction procedures
  • Convenient format - single-tube extraction minimizes contamination risks
  • Proven performance - powered by MyTaq™ HS for fast and highly-specific amplification

Applications

  • Ideal for high-throughput genotyping from mammalian tissues
  • Detection of transgenes
  • Knockout analysis

Instrument Compatibility

Compatible with all standard PCR instruments.

Description

Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail (Fig. 1) or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.

The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Bioline. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield (Fig. 2).

The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization.


Notes

MyTaq is a trademark of Bioline.


Components

Reagent

100 Reactions

500 Reactions

Buffer A

2 x 1ml

10 x 1ml

Buffer B

1 x 1ml

5 x 1ml

MyTaq HS Red Mix, 2x

1 x 1.25ml

5 x 1.25ml


Concentration

2x


QC specifications

Bioline operates under ISO 9001 Management System. MyTaq Extract-PCR Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release.


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

Shipped on Blue Ice.

Certificates of Analysis (COAs)

Certificates of Analysis for MyTaq™ Extract-PCR Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 27 COAs for MyTaq™ Extract-PCR Kit →


Frequently asked questions

  • Is a sample available?

      Please fill-in your information below in order to request your sample. You will receive an email confirmation within two business days with delivery details. 

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  • 1. Which polymerase do I need for my specific application?

      At Bioline we pride ourselves in supplying high-quality polymerases to suit your requirements. To aid your selection of the most suited enzyme for your specific applications, please see our polymerase selection guide.

  • 2. What are the storage conditions and stabilities of Bioline polymerases?

      Polymerases should be stored at -20°C and, when stored under the recommended conditions, will remain stable until the expiry date on the outer box label.
      Please Note: We do not recommend the storage of our polymerases at -80°C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.

  • 3. What do the terms yield, fidelity, processivity and specificity actually mean?

      These terms refer to parameters to be considered when performing PCR, and are important features in selecting the correct enzyme for your needs. Understanding what they mean is therefore crucial:
      • Yield: The amount of DNA produced in a PCR reaction
      • Fidelity: The accuracy of the enzyme at incorporating the correct dNTP to the elongating DNA strand.
      • Processivity: The length of time a polymerase is associated with the template, and therefore the size of fragment which can be amplified.
      • Specificity: A measure of the unwanted by-products generated in a reaction.

  • 4. What is a proofreading polymerase?

      In nature, the ability of a DNA polymerase to correct misincorporations of nucleotides in the DNA strand being elongated is often crucial to the survival of the host organism. This ability is termed “proofreading activity”, and occurs in the 3’ to 5’ direction. This activity also leads to the polymerase removing unpaired nucleotides overhanging at the 3’end (A-overhangs), creating blunt ends.

  • 5. What are the error rates of Bioline DNA Polymerases?

      Please see the following table for the appropriate error rates:

      BIOTAQ, BIOTAQ Red, IMMOLASE, Mango Taq, MyTaq DNA Polymerase  1.1 x 104 base substitutions/bp (Tindall and Kunkel, 1988)
       2.4 x 105 frameshift mutations/bp (Tindall and Kunkel, 1988)
       2.1 x 104 errors/bp (Keohavang and Thilly, 1989)
       7.2 x 105 errors/bp (Ling et al., 1991)
       8.9 x 105 errors/bp (Cariello et al., 1991)
       2.0 x 105 errors/bp (Lundberg et al., 1991)
       1.1 x 104 errors/bp (Barnes, 1992)
      ACCUZYME  1.6 x 106 errors/base (Lundberg et al., 1991).
      VELOCITY  4.4 x 10-7 errors/base (Frey & Suppmann, 1995).
  • 6. How is one unit of activity of a polymerase defined?

      One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid- insoluble form in 30 minutes at 72°C.

  • 7. What are the advantages of using a 2x Mix rather than setting up a PCR reaction from scratch?

      All Bioline polymerases are available in the convenient format of a 2x master mix, containing all the reagents and additives necessary to perform successful PCR, with the exception of template, primers and water.

      This formulation not only provides a convenient and hassle-free PCR setup, but also significantly reduces the chances of human error, inaccuracies and contamination by reducing the pipetting steps required.

  • 8. What is the concentration of the polymerase mixes?

      All Bioline PCR mixes come supplied at a 2x concentration, requiring the user simply to add template, primers and PCR grade water to a final concentration of 1x.

  • 9. What is the recommended reaction volume of the polymerase mixes?

      We recommend a final reaction volume of 50µl, this requiring the use of 25µl of the 2x master mix, and to make up to 50µl using template, primers and PCR grade water.

  • 10. What is the composition of the polymerase mixes?

      Whilst the exact composition of the mixes is proprietary information, all mixes are made up of Reaction Buffer, Magnesium, dNTP's, Polymerase and additives, designed to keep the polymerase stable in the mix, as well as provide the optimal conditions for PCR.



Customer Testimonials

We tested the MyTaq Extract-PCR Kit for genotyping mice.
The extraction was fast, easy to handle and the PCR reactions worked very well.
We used the Taq also for performing multiplex PCR and we obtained better results than with our conventional method.
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Our lab has had such trouble from genotyping. We initially started with a simple hot shot method using our own reagents, but the freeze-thaw cycles were degrading the DNA making them unreliable samples. We then began a phenol chloroform procedure to extract the DNA and got great, high quality DNA, but the time versus cost to do this is a problem. Additionally, even with the purest of DNA, some of the samples had to be run on the thermocycler in duplicate again and again to confirm genotype with the Taq we were using. We have faithfully always purchased Bioline’s HyperLadder, and were interested in the MyTaq Extract-PCR Kit when we found out about it at a supplier expo. This kit is absolutely amazing. We went from a two-day purification procedure down to 15 minutes, and the DNA is reliable and survives freeze-thaw cycles. Most importantly, though, the MyTaq is also extremely reliable, and we only have to run samples once to confirm genotyping - and the gels we get are so clean! We have tested all of our mice strains using this kit and all of them work. We are switching to use this permanently from now on! If you have any trouble at all with some of your genotyping, I would give this kit a try.
"When compared with three other DNA extraction/PCR kits or methods, I found that the MyTaq Extract-PCR kit yielded the highest quality DNA; it was faster and easier to use, much more efficient and reliable."
"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."

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