MyTaq™ HS DNA Polymerase

Cat No. Size List Price* Qty  
BIO-21111 250 Units
$148.00
 
BIO-21112 1000 Units
$531.00
 
BIO-21113 2500 Units
$1,198.00
 
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MyTaq™ HS is a very high-performance, antibody-mediated hot-start DNA polymerase, designed for fast, highly-specific PCR.

MyTaq HS DNA Polymerase has the added convenience of allowing room temperature reaction assembly, without non-specific amplification and primer-dimer formation.

Features & Benefits

  • Universal DNA polymerase - recommended for most applications
  • Fast - complete PCR reaction in less than 30 minutes
  • Antibody-based hot-start polymerase - for highest specificity
  • Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
  • All-in-one mastermix available - for enhance reproducibility

Applications

  • Fast PCR reactions
  • Colony PCR
  • Genotyping
  • Multiplexing
  • Low-copy PCR assays
  • Specific amplification of difficult templates (GC-rich)
  • Assays with prolonged reaction set-up

Description

MyTaq HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, resulting in significant improvements in yield, sensitivity and speed. MyTaq HS has the added convenience of room temperature reaction assembly, thus avoiding non-specific amplification and primer-dimer formation. With activation times as low as 1 to 2 minutes, a complete PCR assay can be completed in less than 30 minutes (fig. 1).

The enzyme is supplied with an industry-leading novel buffer system, consisting of a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations. This removes the need for optimizing individual reactions and delivers superior amplification time after time (fig. 2).


Notes

MyTaq is a trademark of Bioline.


Components

Reagent

250 Units

1000 Units

2500 Units

MyTaq HS DNA Polymerase

1 x 50µl

1 x 200µl

2 x 250µl

5x MyTaq Reaction Buffer

2 x 1ml

8 x 1ml

14 x 1.5ml


Concentration

5u/µl


Storage & Stability

All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.


Shipping conditions

On Dry Ice or Blue Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for MyTaq™ HS DNA Polymerase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 34 COAs for MyTaq™ HS DNA Polymerase →

Citations

  1. A potentially fatal mix of herpes in zoos
    Greenwood A.D., Tsangaras K, Ho S.Y., Szentiks C.A., Nikolin V.M., Ma G., Damiani A., East M.L., Lawrenz A., Hofer H., Osterrieder N.
  2. Interactions among flavonoids of propolis affect antibacterial activity against the honeybee pathogen Paenibacillus larvae
    Mihai C.M., M?rghita? L.A., Dezmirean D.S., Chiril? F., Moritz R.F., Schluns H.
  3. Preparation of Single-Stranded DNA from PCR Products with Streptavidin Magnetic Beads
    Wilson R.
  4. Role of Plasmids in Lactobacillus brevis BSO 464 Hop Tolerance and Beer Spoilage
    Bergsveinson, J., Baecker, N., Pittet, V., Ziola, B.
  5. Adenovirus species c is associated with chronic suppurative lung diseases in children
    Wurzel, D.F., Mackay, I.M., Marchant, J.M., Wang, C.Y., Yerkovich, S.T., Upham, J.W., Smith-Vaughan, H., Petsky, H.L., Chang, A.B.

Frequently asked questions

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Customer Testimonials

"MyTaq HS DNA Polymerase is more robust than Clontech Taq."
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"The DNA isolation we use is not the cleanest and with certain PCR it can be problematic if the Taq is not top quality. We had barely any signal with the other Taq. When we switch to MyTaq, the bands were there and clean! Nice product!"
"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."

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