MyTaq™ HS Mix

Cat No. Size List Price* Qty  
BIO-25045 200 x 50µl Reactions
$301.00
 
BIO-25046 1000 x 50µl Reactions
$1,131.00
 
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*For more information on pricing please contact us

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MyTaq™ HS Mix is a ready-to-use 2x mix for fast, highly-specific, hot-start PCR.

MyTaq HS Mix is powered by antibody mediated hot-start and does not exhibit polymerase activity during room-temperature reaction set-up, thus reducing non-specific amplification.

Features & Benefits

  • All-in-one mastermix formulations - for enhance reproducibility
  • Fast - complete PCR reaction in less than 30 minutes
  • Antibody-based hot-start polymerase - for highest specificity
  • Superior performance - highly optimized buffer system, including ultra-pure dNTPs and MgCl2
  • Available with red dye - for direct gel loading

Applications

  • Fast PCR reactions
  • Colony PCR
  • Genotyping
  • Multiplexing
  • Low-copy PCR assays
  • Specific amplification of difficult templates (GC-rich)
  • Assays with prolonged reaction set-up

Description

MyTaq™ HS Mix is a ready-to-use 2x mix for fast, highly-specific, hot-start PCR. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield (fig. 1), making it ideal for high-throughput.

MyTaq HS Mix contains all the reagents including MyTaq buffer, dNTPs, MgCl2, enhancers and stabilizers necessary for trouble-free PCR reaction set up. The product is supplied conveniently all-in-one tube to reduce the number of pipetting steps and to facilitate increased efficiency, throughput and reproducibility. This makes MyTaq HS Mix perfect for techniques such as multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, even though the amplicons are different sizes (fig. 2).

MyTaq HS Mix enables success in multiplex PCR at the first attempt and can even be used for fast multiplex PCR, without special kits, buffers or optimization.


Notes

MyTaq is a trademark of Bioline.


Components

Reagent

200 Reactions

1000 Reactions

MyTaq HS Mix, 2x

4 x 1.25ml

20 x 1.25ml


Concentration

2x


Storage & Stability

All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.


Certificates of Analysis (COAs)

Certificates of Analysis for MyTaq™ HS Mix are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 28 COAs for MyTaq™ HS Mix →




Customer Testimonials

MyTaq HS Mix (BIO-25045) works great even in samples where other PCR mixes don't amplify and have problems. Simple and reliable.
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"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."

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