MyTaq™ One-Step RT-PCR Kit

Cat No. Size List Price* Qty  
BIO-65048 25 Reactions
BIO-65049 100 Reactions
*For more information on pricing please contact us

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The MyTaq™ One-Step RT-PCR Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube.

A combination of the latest advances in buffer chemistry, together with a reverse transcriptase and hot-start DNA polymerase system, ensure that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers.

Features & Benefits

  • Extremely sensitive - blend of RT and novel MyTaq HS
  • Highly optimized - for detection of low-copy genes
  • Superior performance - overcomes secondary structure in difficult and GC-rich targets
  • High-quality - full-length cDNA from as little as 3pg total RNA
  • Simple to use - all-in-one mix for highly-specific and ultrasensitive products for use in downstream applications


  • Gene-expression analysis
  • Transcription analysis
  • Gene cloning
  • Multiplex RT-PCR


MyTaq™ One-Step RT-PCR Kit uses the latest advances in buffer chemistry combined with a novel reverse transcriptase and hot-start DNA polymerase. This ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive one-step RT-PCR (Fig. 1), perfect for all downstream applications.

MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that collectively create a simple to use all-in-one mix.

The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels. The one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-expression analysis.

The cDNA can be synthesised with starting amounts of RNA template from 3pg to 1μg, over a broad temperature range (up to 50°C (Fig. 2) to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq HS DNA Polymerase.


MyTaq is a trademark of Bioline.



25 Reactions

100 Reactions

MyTaq One-Step mix (2x)

1 x 625µl

2 x 1.25ml

RiboSafe RNase   Inhibitor (10u/µl)

1 x 25µl

1 x 100µl

Reverse transcriptase

1 x 12.5µl

1 x 50µl

DEPC-treated Water

1 x 1.8ml

1 x 1.8ml

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on dry/blue ice

Certificates of Analysis (COAs)

Certificates of Analysis for MyTaq™ One-Step RT-PCR Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 23 COAs for MyTaq™ One-Step RT-PCR Kit →


  1. Estimating the fitness advantage conferred by permissive neuraminidase mutations in recent oseltamivir-resistant A (H1N1) pdm09 influenza viruses
    Butler, J., Hooper, K.A., Petrie, S., Lee, R., Maurer-Stroh, S., Reh, L., Guarnaccia, T., Baas, C., Xue, L., Vitesnik, S., Leang, S.K., McVernon, J., Kelso, A., Barr, I.G., McCaw, J.M., Bloom, J.D., Hurt, A.C.
  2. Differential Responses to Virus Challenge of Laboratory and Wild Accessions of Australian Species of Nicotiana, and Comparative Analysis of RDR1 Gene Sequences
    Wylie, S.J., Zhang, C., Long, V., Roossinck, M.J., Koh, S.H., Jones, M.G., Iqbal, S., Li, H.
  3. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling
    Cen, B., Xiong, Y., Song, J.H., Mahajan, S., DuPont, R., McEachern, K., Kraft, A.S.
  4. Clinical cardiomyopathy syndrome in Atlantic salmon, Salmo salar L
    Rodger, H.D., McCleary, S.J., Ruane, N.M.
  5. Yellow tailflower mild mottle virus and Pelargonium zonate spot virus co?infect a wild plant of red?striped tailflower in Australia
    Li, H., Zhang, C., Luo, H., Jones, M.G. K., Sivasithamparam, K., Koh, S.H., Ong, J.W.L., Wylie, S.J.,

Customer Testimonials

"When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch."

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