Oligo (dT)18

Cat No. Size List Price* Qty  
BIO-38029 27µg
$135.00
 
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Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.

Features & Benefits

  • Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers
  • Binds only to mRNA -can be used for cDNA synthesis with total RNA

Applications

  • cDNA synthesis with a reverse transcriptase

Description

Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.

Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´

A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.


Components

Reagent

Volume

Oligo dT (270ng/µl)

1 x 100µl


Concentration

100µl at 270ng/µl


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

Oligo (dT)18 is shipped on dry/blue ice


Certificates of Analysis (COAs)

Certificates of Analysis for Oligo (dT)18 are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 10 COAs for Oligo (dT)18 →





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