Proteinase K Solution

Cat No. Size List Price* Qty  
BIO-37084 5ml
$190.00
 
BIO-37085 5 x 5ml
$864.00
 
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Proteinase K is a highly active serine protease with broad cleavage specificity on native and denatured proteins. Proteinase K is widely used in the purification of native RNA and DNA from tissues or cell lines and many other applications.

Features & Benefits

  • High activity - broad-spectrum serine protease
  • Active under denaturing conditions - useful for a variety of applications
  • Stable - at high temperatures
  • Molecular biology grade - useful for general digestion of proteins
  • Proteinase K is also available in powder form

Applications

  • Inactivation of RNases/DNases during nucleic acid extraction
  • Protein modification
  • General protein digestion
  • Determination of enzyme localization
  • Remove nucleases for in situ hybridization
  • Improving cloning efficiency of PCR products

Description


Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is especially suitable for isolating PCR and RT-PCR templates.

The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100µg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

Proteinase K products are free of detectable DNase and RNase.


Components

Reagent

BIO-37084

 BIO-37085

Proteinase K Solution (20mg/ml)

5ml

5 x 5ml


Concentration


Proteinase K is available as a powder or as a stabilized ready-to-use 20mg/ml (600mAU/ml) stock solution using a specially formulated calcium acetate buffer.

Storage & Stability

Proteinase K can be stored for 12 months at -20°C.

Shipping conditions


On Dry Ice or Blue Ice.

Certificates of Analysis (COAs)

Certificates of Analysis for Proteinase K Solution are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 26 COAs for Proteinase K Solution →





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