Proteinase K

Cat No. Size List Price* Qty  
BIO-37037 100mg
$140.00
 
BIO-37039 1000mg
$998.00
 
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Proteinase K is a highly active serine protease with broad cleavage specificity on native and denatured proteins.

Proteinase K is widely used in the purification of native RNA and DNA from tissues or cell lines, as well as for many other applications.

Features & Benefits

  • High activity - broad-spectrum serine protease
  • Active under denaturing conditions - useful for a variety of applications
  • Stable - at high temperatures
  • Molecular biology grade - useful for general digestion of proteins
  • Proteinase K is also available as a stabilized stock solution

Applications

  • Inactivation of RNases/DNases during nucleic acid extraction
  • Protein modification
  • General protein digestion
  • Determination of enzyme localization
  • Remove nucleases for in situ hybridization
  • Improving cloning efficiency of PCR products

Description

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates.

The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100μg/ml for protein removal and enzyme inactivation and up to 2mg/ml for tissue treatment.

Proteinase K products are free of detectable DNase and RNase.


Components

Reagent

BIO-37037

 BIO-37039

Proteinase K Powder

100mg

1000mg


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for Proteinase K are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 12 COAs for Proteinase K →





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