qPCR Exogenous Control enables users of diagnostic assay to validate their DNA extraction step. qPCR Exogenous Control is "spiked" into the lysis buffer with the sample prior to DNA extraction.
Following DNA extraction, the reaction mix is added to the extracted DNA prior to amplification. All components required for amplification of sample DNA should also be added. Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.
- Simple - easy monitoring and validation of DNA extraction protocols
- Specific - minimal interference with sample detection
- Optimized - ideal for human samples such as stool
- Sensitive - specially designed for qPCR assays
A common practice in qPCR is to add a known amount of “spiked” control DNA after DNA extraction. This monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Bioline have specially developed qPCR Exogenous Control, comprising DNA Exogenous Control and 50x Control Mix, to validate extraction of bacterial DNA from human samples such as stool. Genetic material from the test sample and the DNA Exogenous Control simultaneously extracted by common extraction methods, with the DNA Exogenous Control being as sensitive to inhibition and extraction failure as the test sample.
The DNA Exogenous Control is spiked into the lysis buffer with the target sample, prior to DNA extraction. The 50x Control Mix is then added to the reaction mix before amplification. Signal derived from the DNA Exogenous Control confirms the success of the extraction step.
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qPCR Exogenous Control is suitable for use with commercially available silica-membrane DNA extraction kits and CHELEX matrices and has been tested on a wide range of qPCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.
qPCR Exogenous Control uses Redmond Red which is a performance-optimized fluorophores for qPCR.
Certification of Analysis (COAs)
DNA Exogenous Control
10 x 400 µL
50x Control Mix
1 x 100 µL
Storage & Stability
All components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date on the outer box label.
Shipped on dry/blue ice.