Quick-Stick Ligase

Cat No. Size List Price* Qty  
BIO-27027 50 Reactions
$211.00
 
BIO-27028 100 Reactions
$358.00
 
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Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature.

Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity and which contains a specially developed 4x Quick-Stick Buffer.

Features & Benefits

  • Fast - dramatically decreases time for DNA cloning
  • Catalyzes the joining of double-stranded DNA - for blunt or cohesive ends
  • Compatible with downstream applications - no loss of transformation efficiency

Applications

  • Cloning of DNA from:
    • PCR products
    • Plasmids
    • Cosmids
    • Genomic, phage and viral DNA
  • Linker ligation
  • Re-ligation of linearized plasmids
  • Ligation of double-stranded oligonucleotides into vectors (plasmid and phage)

Description

Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5’-phosphate and the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration.

Quick-Stick Ligase will ligate 99% of λ/HindIII cohesive-ended fragments, or 80% of λ/EcoRV blunt-ended fragments, in 5 minutes at room temperature. 100% ligation of blunt-ended fragments can be achieved by increasing the ligation time to 15 minutes at room temperature.


Components

Reagent

50 reactions

100 reactions

Quick-Stick Ligase

1 x 50µl

1 x 100µl

4 x QS Buffer

1 x 250µl

2 x 250µl

DNA Dilution Buffer

1 x 1.25ml

1 x 1.25ml


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Blue Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for Quick-Stick Ligase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 13 COAs for Quick-Stick Ligase →





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