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    Cat. No.
    Size
    List Price*
    QTY
    BIO-27027
    50 Reactions
    $217.00
    +
    BIO-27028
    100 Reactions
    $365.00
    +
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    Description

    Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature.

    Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity and which contains a specially developed 4x Quick-Stick Buffer.

    Product Highlights

    • Fast - dramatically decreases time for DNA cloning
    • Catalyzes the joining of double-stranded DNA - for blunt or cohesive ends
    • Compatible with downstream applications - no loss of transformation efficiency

    Product Description

    Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5’-phosphate and the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration.

    Quick-Stick Ligase will ligate 99% of λ/HindIII cohesive-ended fragments, or 80% of λ/EcoRV blunt-ended fragments, in 5 minutes at room temperature. 100% ligation of blunt-ended fragments can be achieved by increasing the ligation time to 15 minutes at room temperature.

    Applications

    • Cloning of DNA from:
    • PCR products
    • Plasmids
    • Cosmids
    • Genomic, phage and viral DNA
    • Linker ligation
    • Re-ligation of linearized plasmids
    • Ligation of double-stranded oligonucleotides into vectors (plasmid and phage)
    Main

    Specification

    Components

    Reagent

    50 reactions

    100 reactions

    Quick-Stick Ligase

    1 x 50 µL

    1 x 100 µL

    4 x QS Buffer

    1 x 250 µL

    2 x 250 µL

    DNA Dilution Buffer

    1 x 1.25 mL

    1 x 1.25 mL

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    On Blue Ice.