Cat No. Size List Price* Qty  
BIO-25052 500 x 50µl Reactions
*For more information on pricing please contact us

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RANGER Mix is a ready-to-use 2x mix, designed for the amplification of templates of 10kb or greater.

The advanced formulation of RANGER Mix enables extreme sensitivity and increased fidelity. For convenience, all of the components necessary for trouble-free PCR reactions are supplied in one tube to reduce the number of pipetting steps and improve efficiency and reproducibility.

Features & Benefits

  • Convenient - all-in-one mastermix for ease of set-up
  • Fast antibody-based hot-start polymerase - for highest specificity and speed
  • Superior performance - unique buffer system, including ultra-pure dNTPs and MgCl2
  • Higher-fidelity than Taq - Suitable for all long range applications


  • Validated for human genomic DNA up to 25kb
  • Suitable for TA cloning


RANGER Mix is a ready-to-use 2x mix, based on the RANGER hot-start enzyme designed to amplify templates up to 25kb with extreme sensitivity (Fig. 1) and increased fidelity. RANGER Mix has the added convenience of room temperature reaction assembly due to its antibody- based hot-start property, this reduces unwanted non-specific amplification such as primer-dimer formation.

RANGER Mix contains all the reagents necessary for trouble-free PCR reaction set- up. For your convenience, all of the components are supplied in one tube, to reduce the number of pipetting steps and to improve efficiency and reproducibility.


Purchase of this product does not convey a licence to perform any patented process.



100 Reactions

500 Reactions

RANGER Mix, 2x

2x 1.25ml

10 x 1.25ml


  • BIO-25051: 100 x 50µl Reactions: 2 x 1.25ml
  • BIO-25052: 500 x 50µl Reactions: 10 x 1.25ml


  • 2x

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.

Shipping conditions

Shipped on Dry Ice or Blue Ice

Certificates of Analysis (COAs)

Certificates of Analysis for RANGER Mix are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 9 COAs for RANGER Mix →

Customer Testimonials

"I was really impressed with how well the RANGER Mix worked on my long range application for screening ES cell colonies and I will be switching over to this immediately."
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"RANGER Mix works well on long PCR products. The fragments I was trying to amplify were both ~10kb and the yield was good for both of them."

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