RiboSafe RNase Inhibitor

Cat No. Size List Price* Qty  
BIO-65027 2500u
$101.00
 
BIO-65028 10,000u
$329.00
 
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RiboSafe RNase Inhibitor is a recombinant protein which inhibits different RNases (A, B, C) by binding non-covalently in a 1:1 ratio. With an association constant of 1014M, RiboSafe RNase Inhibitor is useful in any applications where the presence of RNases is a potential problem.

RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases and exonucleases.

Features & Benefits

  • Complete inhibition - of RNase A, B and C
  • Pure - DNase/RNase and Nickase free
  • Free from contaminants - no inhibition of polymerase/transcriptase activity
  • Stable - over a wide range of pH, temperatures and DTT concentrations

Applications

  • RNA purification
  • cDNA preparation by reverse transcription
  • RNA sequencing
  • in vitro RNA transcription
  • in vitro protein synthesis

Description

Bioline Ribonuclease Inhibitor (RiboSafe RNase Inhibitor) is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C by binding non-covalently in a 1:1 ratio (fig. 1 & 2). RiboSafe shows no inhibition of polymerase (fig. 3) or transcriptase activity (fig. 4) and it is not effective against RNase H, T1, S1-nuclease or RNase from Aspergilus.

With an association constant of 1014M, RiboSafe is useful in any applications where the presence of RNases is a potential problem. RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases and exonucleases. The enzyme is supplied at a concentration of 40u/μl.


Components

Reagent

BIO-65027

BIO-65028

RiboSafe RNase Inhibitor (40u/µl)

62.5µl (2,500u)

250µl (10,000u)


Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on dry/blue ice.


Certificates of Analysis (COAs)

Certificates of Analysis for RiboSafe RNase Inhibitor are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 20 COAs for RiboSafe RNase Inhibitor →





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