RNA Extraction Control 560

Cat No. Size List Price* Qty  
BIO-38044 100 Reactions
$271.00
 
BIO-38045 500 Reactions
$947.00
 
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RNA Extraction Control enables users of diagnostic assays to validate their extraction step. RNA Extraction Control contains a known concentration of artificial cells that contain the control RNA sequence. Cells containing internal control RNA are spiked into the lysis buffer with the sample prior to RNA extraction.

Following extraction, the reaction mix is added to the extracted RNA prior to reverse transcription and amplification. Presence of internal control RNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample RNA.

Features & Benefits

  • Simple - easy monitoring and validation of RNA extraction protocols
  • Specific - minimal interference with sample detection
  • Optimized - ideal for blood, urine and sputum samples
  • Sensitive - specially designed for real-time PCR assays

Instrument Compatibility

RNA Extraction Control is suitable for use with commercially available silica-membrane RNA extraction kits and CHELEX matrices and has been extensively tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

To fit in with existing protocols, RNA Extraction Control 560 uses Cal Fluor® Orange 560 and is also available with Quasar® 670 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.


Description

A common practice in real-time PCR is to add a known amount of “spiked” control RNA after RNA extraction. This monitors PCR inhibition but has no value as an extraction control or indicator of reverse transcription efficiency. RNA Extraction Control (REC) provides quality control assurance for the entire workflow, from sample extraction and  reverse transcription/cDNA synthesis through to real-time amplification and analysis.

The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1). Bioline has specially developed a RNA Extraction Control that more closely mimics the test sample, as compared to “spike” controls. The genetic material from the test sample and REC is simultaneously extracted through common extraction methods, with REC being as sensitive to inhibition and extraction failure as the test sample.

REC consists of artificial cells of a known concentration that contain the Internal Control RNA sequence. This sequence has no known homology to any organism and, importantly, has minimal interference with the detection of sample RNA. REC cells are “spiked” into the lysis buffer with the target sample prior to RNA extraction.

The Control Mix, comprising primers and probe, is then added to the reaction mix prior to amplification. The signal derived from the Internal Control RNA confirms the success of the extraction step (fig. 2). REC also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns (fig. 3).


Components

Reagent

100 Reactions

500 Reactions

2000 Reactions

5000 Reactions

Internal Control RNA

1 x 200 µL

5 x 200 µL

20 x 200 µL

50 x 200 µL

25 x Control Mix

1 x 100 µL

5 x 100 µL

20 x 100 µL

50 x 100 µL


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on dry/blue ice.


Certificates of Analysis (COAs)

Certificates of Analysis for RNA Extraction Control 560 are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 9 COAs for RNA Extraction Control 560 →





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