RNA Extraction Control 670

Cat No. Size List Price* Qty  
BIO-38040 100 Reactions
$271.00
 
BIO-38041 500 Reactions
$947.00
 
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RNA Extraction Control enables users of diagnostic assays to validate their extraction step. RNA Extraction Control contains a known concentration of artificial cells possessing the control RNA sequence. Cells containing internal control RNA are “spiked” into the lysis buffer with the sample prior to RNA extraction.

Following extraction the reaction mix is added to the extracted RNA prior to reverse transcription and amplification. Presence of internal control RNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample RNA.

Features & Benefits

  • Simple - easy monitoring and validation of RNA extraction protocols
  • Specific - minimal interference with sample detection
  • Optimized - ideal for blood, urine and sputum samples
  • Sensitive - specially designed for real-time PCR assays

Instrument Compatibility

RNA Extraction Control is suitable for use with commercially available silica-membrane RNA extraction kits and CHELEX matrices and has been tested on a wide range of real-time PCR platforms including ABI-7500, LightCycler 480®, RotorGene-Q™ and MX3005P®.

RNA Extraction Control 670 uses Quasar® 670 and is also available with Cal Fluor® Orange 560 or Cal Fluor® Red 610, to fit in with existing protocols. CAL Fluor and Quasar dyes are performance-optimized fluorophores for multiplex real-time PCR.


Description

A common practice in real-time PCR is to add a known amount of “spiked” control RNA after RNA extraction. This monitors PCR inhibition but has no value as an extraction control or to show efficiency of reverse transcription. The ideal situation is to have the test sample and internal control undergo the same processing prior to real-time PCR (fig. 1).

Bioline has specially developed RNA Extraction Control (REC) that more closely mimics the test sample, as compared to spike controls. The genetic material from the test sample and the REC are simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.

The REC consists of artificial cells of a known concentration that possess the Internal Control RNA sequence. This sequence contains no known homology to any organism and importantly, has minimal interference with detection of sample RNA. The REC cells are spiked into lysis buffer with the target sample, prior to RNA extraction. Control Mix (primers and probe) is then added to the reaction mix before amplification. Signal derived from the Internal Control RNA confirms the success of the extraction step (fig. 2). REC also monitors co-purification of PCR inhibitors (fig. 3) that may cause biased or false amplification patterns.

REC offers the quality control assurance required for the entire workflow from sample extraction, reverse transcription/cDNA synthesis to real-time amplification and analysis.


Components

Reagent

100 Reactions

500 Reactions

2000 Reactions

5000 Reactions

Internal Control RNA

1 x 200 µL

5 x 200 µL

20 x 200 µL

50 x 200 µL

25 x Control Mix

1 x 100 µL

5 x 100 µL

20 x 100 µL

50 x 100 µL


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on dry/blue ice.


Certificates of Analysis (COAs)

Certificates of Analysis for RNA Extraction Control 670 are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 14 COAs for RNA Extraction Control 670 →





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