SensiFAST™ SYBR® No-ROX Kit

Cat No. Size List Price* Qty  
BIO-98005 500 x 20µl Reactions
$408.00
 
BIO-98020 2000 x 20µl Reactions
$1,484.00
 
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*For more information on pricing please contact us

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SensiFAST SYBR® No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time instruments.

The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and ultra-sensitive real-time PCR.

Features & Benefits

  • Rapid - hot-start capability saves time and reduces primer-dimer formation
  • Unique buffer chemistry - for highest specificity
  • Broad dynamic range - for high sensitivity
  • Simple and reproducible - just add primers and template

Instrument Compatibility

Roche LightCycler® 480 platform, LightCycler® Nano, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q, Eppendorf Mastercycler®ep realplex, PCRmax Eco™, Takara Thermal Cycler Dice® (TP800), Analytik Jena qTOWER and Techne Quantica®, PrimeQ.


Description

SensiFAST SYBR® No-ROX Kit is designed for fast, highly sensitive and reproducible real-time PCR and has been validated on commonly used real-time instruments.

The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation. This allows a much greater dynamic range by removing competition for reaction components during amplification, in turn allowing greater sensitivity. The addition of the latest advances in buffer chemistry and enhancers also ensures that the SensiFAST SYBR® No-ROX Kit produces fast, highly-specific and reproducible (fig. 1) real-time PCR results in under 30 minutes. For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiFAST SYBR® No-ROX Kit for very fast cDNA synthesis and real-time results, without compromising sensitivity or quality.

For added convenience, SensiFAST SYBR® No-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and enhancers.

SensiFAST SYBR® No-ROX is used with real-time PCR instruments that do not require a passive reference.


Notes

SensiFAST is a trademark of Bioline Reagents Ltd.


Components

Reagent

500 x 20 µL reactions

2000 x 20 µL reactions

SensiFAST SYBR® No-ROX mix (2x)

5 x 1 mL

4 x 5 mL

 


Volume

  • BIO-98002: 200 x 20µl Reactions: 2 x 1ml
  • BIO-98005: 500 x 20µl Reactions: 5 x 1ml
  • BIO-98020: 2000 x 20µl Reactions: 4 x 5ml

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.


Shipping conditions

SensiFAST SYBR® No-ROX Kit is shipped on dry/blue ice


Certificates of Analysis (COAs)

Certificates of Analysis for SensiFAST™ SYBR® No-ROX Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 40 COAs for SensiFAST™ SYBR® No-ROX Kit →

Citations

  1. Involvement of the P2X7 Purinergic Receptor and c-Jun N-Terminal and Extracellular Signal-Regulated Kinases in Cyclooxygenase-2 and Prostaglandin E2 Induction by LL-37
    Chotjumlong. P., Bolscher J.G., Nazmi K., Reutrakul V., Supanchart C., Buranaphatthana W., Krisanaprakornkit S.
  2. Gene expression indicates a zone of heterocyst differentiation within the thallus of the cyanolichen Pseudocyphellaria crocata
    Chua J.P., Wallace E.J., Yardley J.A., Duncan E.J., Dearden P.K., Summerfield T.C.
  3. Aerobic and anaerobic microbial activities in the foreland of a receding glacier
    Hofmann K.,Reitschuler C., Illmer P.
  4. Mycobacterium marinum Causes a Latent Infection that Can Be Reactivated by Gamma Irradiation in Adult Zebrafish
    Parikka M., Hammaren M.M., Harjula S.K., Halfpenny N.J., Oksanen K.E., Lahtinen M.J., Pajula E.T., Iivanainen A., Pesu M., Ramet M.
  5. Methanogenic potential of formate in thermophilic anaerobic digestion
    Lins P., Schwarzenauer T., Reitschuler C., Wagner A.O. and Illmer P.

Frequently asked questions

  • What is the advantage of working with SYBR Green I?

      SYBR Green I is an inexpensive, universal dye which binds to all dsDNA. It can be easily used in combination with a simple primer pair to detect PCR products in real-time. This dye is very attractive for researchers analysing lots of different genes.

  • What is the advantage of working with a probe system?

      The probe system is always specific; only detect the gene of interest. It is also possible to distinguish between similar sequences with small differences like SNPs or mutations. In general, probe assays need less optimisation than SYBR Green I assays.

  • Can I use a SensiFAST Kit for standard real-time PCR?

      Although SensiFAST kits have been designed for fast PCR on the new generation of fast machines, they will work equally well for standard or fast PCR protocols on all real-time PCR machines.
  • Can I use a SensiFAST SYBR Kit for a probe assay?

      This is not possible because the SYBR is pre-mixed into the SensiFAST SYBR mastermix.
  • Why do the SensiFAST kits contain a hot-start Taq polymerase?

      Polymerase activity during the reaction set-up causes non-specific amplification including primer-dimer formation. To avoid non-specific amplification the polymerase is only activated after heating at 95°C for 2-3 minutes.
  • Why do you no longer sell kits contain dUTP (and UNG)?

      This is because it is because it is only effective if all the researchers either doing PCR in the laboratory or using the same thermocycler are also using a dUTP/dNTP and UNG system. If even one researcher is not, it ceases to be an effective control. Using dUTP has also been shown to be inefficient as it increases the Ct values by reducing reaction efficiency. Most labs do not need to use the dUTP method of control, optimised protocols will allow high specificity of PCR and good lab practices (using disposable consumables, the use of filter tips and maintaining a separate area for PCR set-up and PCR amplification and any post-PCR analysis) virtually eliminate the risk of cross contamination.
  • Why do certain kits contain a ROX passive reference?

      The emission recorded from ROX during the baseline cycles is used to normalize the emission recorded from the reporter (SYBR) in later cycles in some instruments. ROX compensates for small fluorescent fluctuations such as bubbles and well-to-well variations that may occur. Each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off.
  • Why are the ROX concentrations different?

      The amount of the ROX passive reference dye needed varies depending on the instrument optics, our SensiFAST kits have been optimised for these different instruments (see Product Selection Tool).
  • What is the difference between using ROX and using fluorescein?

      Fluorescein is an alternative passive reference dye used just in the Bio-Rad instruments (see Product Selection Tool), the SensiFAST SYBR & Fluorescein contains fluorescein premixed in the mastermix at optimised concentrations.
  • Is the SYBR in a separate tube?

      For your convenience the SensiFAST kits have an optimised amount of SYBR Green in the mastermix.
  • What is the difference between a one-step and a two-step real-time PCR reaction?

      In a one-step reaction the reverse transcription reaction and the real-time PCR reaction are done in one tube, making this a closed tube assay, so contamination can be avoided. It saves pipeting steps and time, and is easy in handling, making it ideal for high throughput screening.

      In a two-step reaction the reverse transcription reaction and the real-time PCR reaction are done in separate tubes. It gives a more flexible way of working in that the cDNA can be used for more than one real-time PCR reaction and can be archived, eliminating the need to continually isolate RNA. For convenience Bioline sells the SensiFAST cDNA Synthesis Kit separately if you wish to take a two-step approach.
  • What template (RNA/cDNA) is this compatible with?

      SensiFAST One-Step Kits can be used with most RNA/cDNA templates. To give you some idea of a few of the types of templates used with these kits:

      Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
      Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
      Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
      Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
      Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
      Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
      Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)

      For cDNA/DNA templates SensiFAST SYBR and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:

      Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
      Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
      Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
      Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
      Insect - Bass C., et al. Malaria journal 6 111 (2007)
      Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
      Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
      Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
      Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
      Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
      E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
      Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)


Customer Testimonials

We use the SensiFAST SYBR No-ROX Kit and the Rotor-Gene 6000 for gene expression studies on fruit crops. We tested different kits and the Bioline kit has excellent sensitivity and a competitive price level.
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Amazing! Just take a look to the comparison I have made! And I did some others as well: thaw/freeze cycles (4 times without any change), primers specificities (no changes). All the tests were free using small aliquots from Bioline.
Only one thing would still stop you? The price is the lowest I've ever had. To me, using my conditions and all the tests I ran, this is the best mix for qPCR!
Excellent amplification of Oct4 in induced pluripotent stem cells from 74ng/rx to 0.9ng/rx on Roche Lightcycler 480 with an efficiency of 1.98.
We tested the SensiFAST™ SYBR® No-ROX Kit against the LightCycler® 480 SYBR Green I Master on a Roche Lightcycler 480. The cycling conditions we used were optimized for the Roche kit. Despite this, the SensiFAST kit performed significantly better, leasing to earlier amplification from comparable genomic DNA samples (see picture for example). This gives us the increased sensitivity we were seeking. On top of that it is much cheaper than the Roche mix. I highly recommend this product.
We are using the Bioline SensiFAST SYBR No-Rox Mix for our qPCR studies. We have compared the mix to the Roche Lighcycler 480 SYBR Green I Master Mix. With the Bioline SensiFAST mix, our targets regularly appear at lower Cp values and all primers tested work more reliably and consistently (see attached table for two examples of primer efficiencies determined with the Lightcycler and the SensiFast kits). Furthermore, Bioline technical support always answered our queries very promptly and in great detail and the SensiFAST mix costs us less than half the price of the Lightcycler Mix.
"I have been using a Bioline's qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) for four years with Arabidopsis cDNA on a Bio-Rad CFX-96 and a Roche LightCycler-96 in 15 or 20 ul reaction volumes. I can highlight excellent technical replication, low baseline noise and good signal/noise ratio. Reasonable price quotations allow me also to stretch my budget. I know then that with an appropriate experimental design I can trust my results and expect them on time!"
"Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel."
"Great product, worked exactly how expected, and sensitive to small amounts of starting template."
"SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the difference between a successful and an unsuccessful experiment."
"In the course of optimizing real-time PCR for analysis of various animal tissues, I tested multiple real-time master mixes of different brands. The SensiFAST™ SYBR No-ROX Kit produced the most valid amplification result by improving efficiency and reproducibility. With this product, Bioline offers an optimal cost-performance ratio."

Other researchers use:


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