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    Cat. No.
    Size
    List Price*
    QTY
    BIO-83005
    500 Reactions
    $1,010.00
    +
    BIO-83020
    2000 Reactions
    $3,700.00
    +
    *To check your pricing please Login or contact us

    Description

    Outstanding assay reproducibility, sensitivity and specificity for both DNA and cDNA templates.

    Product Highlights

    • Sensitive – reproducible detection of low-copy number templates
    • Specific – proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
    • Reproducible – consistent results between technical replicates ideal for multiplexing and gene expression analysis
    • Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types

    Product Description

    The SensiMix™ II Probe Kit has been developed for highly accurate real-time PCR and has been validated on all real-time PCR platforms and includes a separate vial of the passive reference dye ROX for normalization of well-to-well differences, if required.

    A combination of the latest advances in buffer chemistry and PCR enhancers with a chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix II Probe Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis and multiplexing.

    The SensiMix II Probe Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

    Applications

    • Gene expression analysis
    • DNA / cDNA target detection
    • miRNA profiling / quantification
    • Copy number variation (CNV) analysis
    Main
    Highly efficient qPCR under standard conditions.

    With SensiMix II Probe Kit I get good melting curves, close Ct values for triplicates and high PCR efficiency. The melting curve shows very low or no primer dimer.

    Fansuo Geng, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

    Instrument Compatibility

    Please refer to the Real-Time PCR Selection Chart to confirm the SensiMix II Probe Kit is compatible with your instrument.


    Specification

    Components

    Reagent

    500 reactions

    2000 reactions

    SensiMix™ II Probe No-ROX (2x)

    10 x 1.25 mL (12.5 mL)

    40 x 1.25 mL (50 mL)

     25 µM ROX Dye

    500 µL

    4 x 500 µL

    50 mM MgCl2

    1 x 1 mL

    1 x 1 mL

    Volume

    BIO-83005: 500 x 50µL Reaction: 10 x 1.25 mL

    BIO-83005: 2000 x 50µL Reaction: 0 x 1.25 mL

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    SensiMix II Probe No-ROX Kit is shipped on dry/blue ice



    Reviews

    "With SensiMix II Probe Kit I get good melting curves, close Ct values for tripli ..."

    "I have reviewed numerous real-time PCR master mixes and I would like to share wi ..."