SensiMix™ II Probe Kit

Cat No. Size List Price* Qty  
BIO-83005 500 Reactions
$980.00
 
BIO-83020 2000 Reactions
$3,596.00
 
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The SensiMix™ II Probe Kit is a high-performance reagent designed for greater sensitivity and specificity, it does not contain a passive reference and is suitable for those block-based and rotor-based machines where an internal reference is not required for normalization. The kit delivers superior performance in gene expression analysis and multiplexing and uses hot-start DNA polymerase to ensure fast, highly-specific and ultra-sensitive real-time PCR, generating reliable and highly reproducible data.

Features & Benefits

  • Superior PCR efficiency - with sequence specific probes
  • Higher specificity - perfect for low copy number samples
  • Simple and reproducible - ideal for multiplexing and gene expression analysis
  • Flexible - compatibility with all real-time PCR instruments

Instrument Compatibility

The SensiMix II Probe Kit can be used on all real-time PCR instruments.


Description

The SensiMix™ II Probe Kit is a high-performance reagent designed for greater sensitivity and specificity. The kit has been formulated for use with probe-detection technology, including hydrolysis probes (e.g. TaqMan®), displacement probes (e.g. Molecular Beacons, Scorpion®) and hybridization probes (e.g. FRET), delivering superior performance in gene expression analysis (Fig. 1) and multiplexing (Fig. 2).

SensiMix II Probe Kit employs a hot-start DNA polymerase for high PCR specificity and sensitivity. SensiMix possesses no polymerase activity during reaction set-up, thereby greatly reducing non-specific amplification, including primer-dimer formation, after pre-heating SensiMix becomes fully activated and in conjunction with specially optimized buffer chemistry, generates reliable and highly reproducible data on all real-time PCR instruments.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiMix II Probe Kit for very high-quality first strand cDNA synthesis and real-time results.

For ease-of-use, SensiMix II Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs and stabilizers.


Notes

SensiMix is a trademark of Bioline Reagents Ltd.


Components

Reagent

500 x 50 µL reactions

2000 x 50 µL reactions

SensiMix™ II Probe (2x)

10 x 1.25 mL (12.5 mL)

40 x 1.25 mL (50 mL)

 25 µM ROX Dye

 500 µL

 4 x 500 µL

50 mM MgCl2

1 x 1 mL

1 x 1 mL


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

SensiMix II Probe Kit is shipped on dry/blue ice


Certificates of Analysis (COAs)

Certificates of Analysis for SensiMix™ II Probe Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 8 COAs for SensiMix™ II Probe Kit →






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