SureClean Plus

Cat No. Size List Price* Qty  
BIO-37047 5ml
$179.00
 
BIO-37048 25ml
$780.00
 
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SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.

SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests with excellent recovery rates.

SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.

Features & Benefits

  • Rapid - column-free PCR clean-up
  • Efficient - post-PCR recovery up to 98%
  • Optional pink dye - for improved visibility and minimal pellet loss
  • Pure - isolated products are suitable for downstream applications

Applications

  • Removes primers, non-specifics, dNTPs and enzymes
  • DNA or dsRNA purification or concentration
  • Buffer exchange

Description

SureClean Plus is a novel, inexpensive solution that provides a column-free method for nucleic acid purification.

Using a simple and rapid procedure, SureClean Plus can be used to purify or concentrate DNA or dsRNA from PCR reactions or any enzymatic digests. This method is easy to follow, combining convenience, speed and excellent recovery rates.

A very straightforward protocol allows the precipitation of nucleic acids ≥75bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns (fig. 1). SureClean Plus purifies nucleic acids without the use of chaotropic salts that often contribute to denaturation of the DNA duplex.

SureClean Plus enables the researcher to re-suspend the cleaned-up nucleic acids in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit your downstream experiments.

Optional Pink Co-Precipitant
SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids.The co-precipitant does not interfere with downtown applications and so SureClean Plus is suitable for use in all cloning, standard PCR, real-time PCR and other enzymatic reactions.


Notes

Nucleic acids purified with SureClean Plus are not suitable for spectral determination of nucleic acids within the 260nm - 280nm range. Should you wish to carry out such experiments, the use of SureClean solution without pink co-precipitant is recommended.


Components

Reagent

5ml

25ml

SureClean

1 x 5ml

2 x 12.5ml

Co-Precipitant Pink

1 x 0.8ml

2 x 2ml


Storage & Stability

All components should be stored at room temperature. Do not freeze. Avoid exposure of Co-Precipitant Pink to light.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.





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